Anion separations with pressure-assisted capillary electrophoresis using a sequential injection analysis manifold and contactless conductivity detection
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 1007364
Author(s) Mai, Thanh Duc; Hauser, Peter C
Author(s) at UniBasel Hauser, Peter C.
Mai, Thanh Duc
Year 2011
Title Anion separations with pressure-assisted capillary electrophoresis using a sequential injection analysis manifold and contactless conductivity detection
Journal Electrophoresis
Volume 32
Number 21
Pages / Article-Number 3000-7
Keywords Anions, Capacitively coupled contactless conductivity detection (C(4)D), Electroosmotic flow compensation, Pressure-assisted capillary electrophoresis (PACE), Sequential injection analysis (SIA)
Abstract It is demonstrated that a hydrodynamic flow superimposed on the mobility of analyte anions can be used for the optimization of analysis time in capillary zone electrophoresis. It was also possible to use the approach for counter-balancing the electroosmotic flow and this works as well as the use of surface modifiers. To avoid any band-broadening due to the bulk flow narrow capillaries of 10 mu M internal diameter were employed. This was enabled by the use of capacitively coupled contactless conductivity detection, which does not suffer from the downscaling, and detection down to between 1 and 20 mu M for a range of inorganic and small organic anions was found feasible. Precisely controlled hydrodynamic flow was generated with a sequential injection manifold based on a syringe pump. Sample injection was carried out with a new design relying on a simple piece of capillary tubing to achieve the appropriate back-pressure for the required split-injection procedure.
Publisher Wiley-VCH
ISSN/ISBN 0173-0835
edoc-URL http://edoc.unibas.ch/dok/A6001929
Full Text on edoc No
Digital Object Identifier DOI 10.1002/elps.201100200
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/21997519
ISI-Number WOS:000298101000010
Document type (ISI) Journal Article
 
   

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