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PAMAM6 dendrimers and DNA : pH dependent "beads-on-a-string" behavior revealed by small angle X-ray scattering
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 967506
Author(s) Dootz, Rolf; Toma, Adriana Cristina; Pfohl, Thomas
Author(s) at UniBasel Pfohl, Thomas
Year 2011
Title PAMAM6 dendrimers and DNA : pH dependent "beads-on-a-string" behavior revealed by small angle X-ray scattering
Journal Soft matter
Volume 7
Number 18
Pages / Article-Number 8343-8351
Abstract DNA interactions with polycations are not only important for our understanding of chromatin compaction but also for characterizing DNA-binding proteins involved in transcription, replication and repair. DNA is known to form several types of liquid-crystalline phases depending, among other factors, on the polycation structure and charge density. Theoretical studies and simulations have predicted the wrapping of DNA around spherical positively charged polycations. As a potential mimic of the histone octamer or other DNA wrapping proteins, poly(amido amine) generation 6 (PAMAM6) dendrimers have been chosen for our study. The self-assembly of DNA induced by PAMAM6 has been investigated using small angle X-ray scattering (SAXS) in order to reveal the assemblies' structure dependence on the pH of the environment and dendrimer concentrations. We demonstrate that at pH 8.5 dense phases are formed and characterized by a 2D-columnar hexagonal lattice which is transformed into a 3D hexagonal lattice with increasing dendrimer concentration (charge ratio N/P). Moreover, a systematic analysis of the scattering data collected from diluted samples at pH 8.5 and 5.5 has led us to propose a wrapping scenario of DNA around PAMAM6 at pH 5.5.
Publisher Royal Society of Chemistry
ISSN/ISBN 1744-683X
edoc-URL http://edoc.unibas.ch/dok/A5848980
Full Text on edoc No
Digital Object Identifier DOI 10.1039/c1sm05632h
ISI-Number WOS:000294447600060
Document type (ISI) Article
 
   
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09/06/2024