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Synergy of repression and silencing gradients along the chromosome
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 961800
Author(s) Ratna, Prasuna; Scherrer, Simone; Fleischli, Christoph; Becskei, Attila
Author(s) at UniBasel Becskei, Attila
Year 2009
Title Synergy of repression and silencing gradients along the chromosome
Journal Journal of Molecular Biology
Volume 387
Number 4
Pages / Article-Number 826-39
Mesh terms Chromosomes, Fungal, genetics; DNA-Binding Proteins, metabolism; Gene Expression Regulation, Fungal; Gene Regulatory Networks; Gene Silencing; Genes, Fungal; Models, Genetic; Repressor Proteins, metabolism; Saccharomyces cerevisiae, metabolism; Saccharomyces cerevisiae Proteins, metabolism; Silent Information Regulator Proteins, Saccharomyces cerevisiae, metabolism; Telomere, genetics
Abstract The expression of a gene is determined by the transcriptional activators and repressors bound to its regulatory regions. It is not clear how these opposing activities are summed to define the degree of silencing of genes within a segment of the eukaryotic chromosome. We show that the general repressor Ssn6 and the silencing protein Sir3 generate inhibitory gradients with similar slopes over a transcribed gene, even though Ssn6 is considered a promoter-specific repressor of single genes, while Sir3 is a regional silencer. When two repression or silencing gradients flank a gene, they have a multiplicative effect on gene expression. A significant amplification of the interacting gradients distinguishes silencing from repression. When a silencing gradient is enhanced, the distance-dependence of the amplification changes and long-range effects are established preferentially. These observations reveal that repression and silencing proteins can attain different tiers in a hierarchy of conserved regulatory modes. The quantitative rules associated with these modes will help to explain the co-expression pattern of adjacent genes in the genome.
Publisher Elsevier
ISSN/ISBN 0022-2836 ; 1089-8638
edoc-URL http://edoc.unibas.ch/46409/
Full Text on edoc No
Digital Object Identifier DOI 10.1016/j.jmb.2009.02.025
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/19233208
ISI-Number WOS:000265302700004
Document type (ISI) Journal Article
 
   

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