4D APSY-HBCB(CG)CDHD experiment for automated assignment of aromatic amino acid side chains in proteins
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 855106
Author(s) Krähenbühl, Barbara; Hiller, Sebastian; Wider, Gerhard
Author(s) at UniBasel Hiller Odermatt, Sebastian
Year 2011
Title 4D APSY-HBCB(CG)CDHD experiment for automated assignment of aromatic amino acid side chains in proteins
Journal Journal of biomolecular NMR
Volume 51
Number 3
Pages / Article-Number 313-8
Keywords Protein NMR, Aromatic resonances, Projection spectroscopy, APSY, GAPRO, Automated assignment
Abstract

A four-dimensional (4D) APSY (automated projection spectroscopy)-HBCB(CG)CDHD experiment is presented. This 4D experiment correlates aromatic with aliphatic carbon and proton resonances from the same amino acid side chain of proteins in aqueous solution. It thus allows unambiguous sequence-specific assignment of aromatic amino acid ring signals based on backbone assignments. Compared to conventional 2D approaches, the inclusion of evolution periods on (1)H(?) and (13)C(?) efficiently removes overlaps, and provides two additional frequencies for consequent automated or manual matching. The experiment was successfully applied to three proteins with molecular weights from 6 to 13 kDa. For the complementation of the assignment of the aromatic resonances, TOCSY- or COSY-based versions of a 4D APSY-HCCH(aro) sequence are proposed.

Publisher Springer
ISSN/ISBN 0925-2738
edoc-URL http://edoc.unibas.ch/dok/A6001520
Full Text on edoc No
Digital Object Identifier DOI 10.1007/s10858-011-9572-7
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/21947871
ISI-Number WOS:000295988200011
Document type (ISI) Journal Article
 
   

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