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B/T cell ratio of rabbit peripheral blood lymphocytes : influence of separation technique on results
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 789180
Author(s) Speck, B.
Author(s) at UniBasel Matter-Walstra, Klazien
Year 1985
Title B/T cell ratio of rabbit peripheral blood lymphocytes : influence of separation technique on results
Journal Journal of immunological methods
Volume 79
Number 1
Pages / Article-Number 143-7
Keywords Animals; B-Lymphocytes/classification; Cell Separation; Female; Fluorescent Antibody Technique; Leukocyte Count; Lymphocytes/*classification; Male; Methods; Rabbits; T-Lymphocytes/classification; Temperature
Abstract Spontaneous in vitro T cell rosette formation at room temperature leading to enrichment of B cells has been reported. We tested 10 individual New Zealand White rabbits sequentially, separating the lymphocytes either at room temperature or at 37 degrees C. T cells are lost constantly at room temperature but to a lesser extent at 37 degrees C. The determination of the yield of lymphocytes after Ficoll separation gives the best control for the accuracy of the results. If lymphocytes are separated at 37 degrees C and if the yield of lymphocytes is greater than 45%, the variation in T cells is small and their number is constant between 62 and 74%. These data show that the reported wide range of T cells in rabbit peripheral blood is due to methodological errors and not inherent in the rabbit.
Publisher Elsevier
ISSN/ISBN 0022-1759
URL http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=3889161
edoc-URL http://edoc.unibas.ch/dok/A5844547
Full Text on edoc No
Digital Object Identifier DOI 10.1016/0022-1759(85)90400-4
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/3889161
ISI-Number 3889161
Document type (ISI) Journal Article
 
   

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