A Flow Cytometry-based Assay for Screening FimH Antagonists
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 765881
Author(s) Scharenberg, Meike; Abgottspon, Daniela; Cicek, Evelin; Jiang, Xiaohua; Schwardt, Oliver; Rabbani, Said; Ernst, Beat
Author(s) at UniBasel Ernst, Beat
Scharenberg, Meike
Abgottspon, Daniela
Jiang, Xiaohua
Schwardt, Oliver
Rabbani, Said
Year 2011
Title A Flow Cytometry-based Assay for Screening FimH Antagonists
Journal Assay and Drug Development Technologies
Volume 9
Number 5
Pages / Article-Number 455-464
Abstract Urinary tract infections (UTIs), including cystitis and pyelonephritis, affect a large proportion of the population and account for significant medical costs. In more than 80% of UTIs, uropathogenic Escherichia coli (UPEC) is the causative pathogen. The initial step in the pathogenesis of the infection is the adherence of UPEC to the human bladder epithelium, enabling the invasion into the host cells and the development of UTIs. This process is mediated by the lectin FimH located on type I pili and enables UPECs to attach to oligomannosides of the glycoprotein uroplakin Ia presented on uroepithelial cells. FimH antagonists such as alpha-D-mannopyranosides have been shown to interfere with the attachment of UPEC to their host cells, thus providing a novel therapeutic opportunity for the treatment and prevention of UTIs. In this article, we report a flow cytometry-based assay to evaluate the potential of FimH antagonists for the prevention of the infection of the human urinary bladder cell line 5637 by UPEC strain UTI89. The assay was optimized and validated, and the inhibitory potency of different a-D-mannopyranosides was determined. Finally, the IC50 values measured by the flow cytometry-based assay were compared with those reported for other assay formats.
Publisher Mary Ann Liebert
ISSN/ISBN 1540-658X ; 1557-8127
edoc-URL http://edoc.unibas.ch/dok/A6001506
Full Text on edoc No
Digital Object Identifier DOI 10.1089/adt.2010.0357
ISI-Number WOS:000295324500002
Document type (ISI) Article
 
   

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