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Analysis of cell surface proteome changes via label-free, quantitative mass spectrometry
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 761309
Author(s) Schiess, Ralph; Mueller, Lukas N.; Schmidt, Alexander; Mueller, Markus; Wollscheid, Bernd; Aebersold, Ruedi
Author(s) at UniBasel Schmidt, Alexander
Year 2009
Title Analysis of cell surface proteome changes via label-free, quantitative mass spectrometry
Journal Molecular and Cellular Proteomics
Volume 8
Number 4
Pages / Article-Number 624-38
Keywords Isotope Labeling, Cell Line, Glycoproteins, Receptor: Insulin, Drosophila Proteins, Reproducibility of Results, Cell Membrane, Animals, Chromatography: Liquid, Endocytosis, Drosophila melanogaster, Insulin, Proteome, Mass Spectrometry
Abstract We present a mass spectrometry-based strategy for the specific detection and quantification of cell surface proteome changes. The method is based on the label-free quantification of peptide patterns acquired by high mass accuracy mass spectrometry using new software tools and the cell surface capturing technology that selectively enriches glycopeptides exposed to the cell exterior. The method was applied to monitor dynamic protein changes in the cell surface glycoproteome of Drosophila melanogaster cells. The results led to the construction of a cell surface glycoprotein atlas consisting of 202 cell surface glycoproteins of D. melanogaster Kc167 cells and indicated relative quantitative changes of cell surface glycoproteins in four different cellular states. Furthermore we specifically investigated cell surface proteome changes upon prolonged insulin stimulation. The data revealed insulin-dependent cell surface glycoprotein dynamics, including insulin receptor internalization, and linked these changes to intracellular signaling networks.
Publisher American Society for Biochemistry and Molecular Biology
ISSN/ISBN 1535-9476 ; 1535-9484
edoc-URL http://edoc.unibas.ch/45911/
Full Text on edoc No
Digital Object Identifier DOI 10.1074/mcp.M800172-MCP200
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/19036722
ISI-Number WOS:000265098200004
Document type (ISI) Journal Article
 
   

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