A GC-MS⁄GC-IRMS for the molecular-level analyses of organic matter and the isotopic characterization of inorganic and organic compounds in aquatic and terrestrial ecosystems (2008)
Third-party funded project
Project title A GC-MS⁄GC-IRMS for the molecular-level analyses of organic matter and the isotopic characterization of inorganic and organic compounds in aquatic and terrestrial ecosystems (2008)
Principal Investigator(s) Lehmann, Moritz
Co-Investigator(s) Boller, Thomas
Alewell, Christine
Kuhn, Nikolaus J.
Niemann, Helge
Organisation / Research unit Departement Umweltwissenschaften / Geochemie Stoffkreisläufe (Lehmann)
Project start 01.07.2008
Probable end 30.06.2009
Status Completed
Abstract

A GC-MS/GC-IRMS for the molecular-level analyses of organic matter and the isotopic characterization of inorganic and organic compounds in aquatic and terrestrial ecosystems

Stable isotopes represent a valuable means to constrain specific biogeochemical processes in natural environments. The main objective of the project is to develop an Environmental Laboratory for Aquatic and Terrestrial Biogeochemistry, which will support research that will make use of molecular-level characterization and (compound-specific) isotope ratio determination methods to assess a wide spectrum of biogeochemical transformations in the natural environment and in the laboratory, and to study sources and the fate of organic compounds in marine, freshwater, and soil systems.The requested infrastructure includes the following instruments:

•    Gas Chromatograph coupled to a quadrupole mass spectrometer (MS) with two detectors (FID and MS) for organic compound recognition. Through the GC-C III interface, the GC will be coupled to the IRMS for isotope measurements on single target molecules.
•    Gas source stable isotope ratio mass spectrometer (IRMS), Gasbench-II Interface, accessories for 18O and H/D analysis in GC eluates, and autosampler for high-precision isotope ratio determination.

One of the major goals of the projects that make use of the new instrumental capacities is to understand the interactions of the bio- with the geo- and atmosphere with respect to the transport of organic materials and the transformation of   inorganic and organic compounds by plants and microorganisms, which is of ultimate importance for the reduction of green house gases and the cycling of elements in aquatic and terrestrial ecosystems. A prime objective will be to use stable isotope measurements to constrain specific biogeochemical and metabolic processes, as well as sources, transport and degradation of organic matter in aquatic and terrestrial environments, and, in turn, to use these constraints to obtain improved estimates on global and regional C and N fluxes, in modern environments as well as in the past.

Planned research themes include: I) using lipid biomarker approaches to study microbial communities in various marine and freshwater environments, II) characterizing organic matter components in lacustrine sediments as well as in soils, and identifying the mechanisms responsible for the longer-term sequestration and degradation of organic C and the emission of greenhouse gases, III) understanding the metabolic pathways of C and N during the symbiosis between microorganisms and plants, and IV) generating data sets for the isotopic composition of nitrate and other N species in various aquatic systems in order to characterize the controls on isotope fractionation of specific N cycle reactions. The spectrum of investigated ecosystems will range from Swiss soil and groundwater systems and small lakes to hydrothermal vent systems in the deep ocean, and the spatial scale of the planned research extends from enzyme biogeochemistry to ocean-scale circulation.

Planned projects address a diverse range of topics, yet they share the common goal of using biomarker and isotope analyses to address important and innovative questions regarding a) the cycling of C and N in terrestrial and aquatic systems on the organism-level, regional and global scales, b) the role of microbes for greenhouse gas emissions and c) the use of C and N stable isotope ratios as environmental tracers, in modern environments and in the past.

Keywords isotopes, organic molecules, compound-specific isotope measurements, nitrogen cycle, carbon cycle
Financed by Swiss National Science Foundation (SNSF)

Published results ()

  ID Autor(en) Titel ISSN / ISBN Erschienen in Art der Publikation
86940  Alewell, C.; Schaub, M.; Conen, F.  A method to detect soil carbon degradation during soil erosion    Biogeosciences  Publication: JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift) 
86879  Conen, F.; Zimmermann, M.; Leifeld, J.; Seth, B.; Alewell, C.  Relative stability of soil carbon revealed by shifts in delta N-15 and C:N ratio  1726-4170  Biogeosciences  Publication: JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift) 
86870  Schaub, Monika; Alewell, Christine  Stable carbon isotopes as an indicator for soil degradation in an alpine environment (Urseren Valley, Switzerland)  1097-0231  Rapid communications in mass spectrometry  Publication: JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift) 
86880  Conen, F.; Karhu, K.; Leifeld, J.; Seth, B.; Vanhala, P.; Liski, J.; Alewell, C.  Temperature sensitivity of young and old soil carbon - Same soil, slight differences in C-13 natural abundance method, inconsistent results  0038-0717  Soil Biology & Biochemistry  Publication: JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift) 
86869  Schaub, Monika; Seth, Barbara; Alewell, Christine  Determination of delta O-18 in soils : measuring conditions and a potential application  1097-0231  Rapid communications in mass spectrometry  Publication: JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift) 
57317  Bourbonnais, Annie; Lehmann, Moritz F.; Waniek, Joanna J.; Schulz-Bull, Detlef E.  Nitrate isotope anomalies reflect N-2 fixation in the Azores Front region (subtropical NE Atlantic)  2169-9275  Journal of Geophysical Research. C, Oceans  Publication: JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift) 
1469102  Bourbonnais, Annie; Lehmann, Moritz F.; Butterfield, David A.; Juniper, S. Kim  Subseafloor nitrogen transformations in diffuse hydrothermal vent fluids of the Juan de Fuca Ridge evidenced by the isotopic composition of nitrate and ammonium  1525-2027  Geochemistry, geophysics, geosystems  Publication: JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift) 
1469107  Walder, Florian; Niemann, Helge; Natarajan, Mathimaran; Lehmann, Moritz F; Boller, Thomas; Wiemken, Andres  Mycorrhizal Networks : Common Goods of Plants Shared under Unequal Terms of Trade  0032-0889  Plant physiology  Publication: JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift) 
1469216  Niemann, H.; Stadnitskaia, A.; Wirth, S. B.; Gilli, A.; Anselmetti, F. S.; Sinninghe Damsté, J. S.; Schouten, S.; Hoppmans, E. C.; Lehmann, M. F.  Bacterial GDGTs in Holocene sediments and catchment soils of a high Alpine lake: application of the MBT/CBT-paleothermometer  1814-9324 ; 1814-9332  Climate of the Past  Publication: JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift) 
1742243  Wenk, Christine B.; Blees, Jan; Zopfi, Jakob; Veronesi, Mauro; Bourbonnais, Annie; Schubert, Carsten J.; Niemann, Helge; Lehmann, Moritz F.  Anaerobic ammonium oxidation (anammox) bacteria and sulfide-dependent denitrifiers coexist in the water column of a meromictic south-alpine lake  0024-3590  Limnology and Oceanography  Publication: JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift) 
2270311  Wirth, Stefanie B.; Gilli, Adrian; Niemann, Helge; Dahl, Tais W.; Ravasi, Damiana; Sax, Nadja; Hamann, Yvonne; Peduzzi, Raffaele; Peduzzi, Sandro; Tonolla, Mauro; Lehmann, Moritz F.; Anselmetti, Flavio S.  Combining sedimentological, trace metal (Mn, Mo) and molecular evidence for reconstructing past water-column redox conditions : the example of meromictic Lake Cadagno (Swiss Alps)  0016-7037  Geochimica et cosmochimica acta  Publication: JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift) 
2270313  Walder, Florian; Niemann, Helge; Lehmann, Moritz F.; Boller, Thomas; Wiemken, Andres; Courty, Pierre-Emmanuel  Tracking the carbon source of arbuscular mycorrhizal fungi colonizing C-3 and C-4 plants using carbon isotope ratios (delta C-13)  0038-0717  Soil biology & biochemistry  Publication: JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift) 
2270320  Brunner, Benjamin; Contreras, Sergio; Lehmann, Moritz F; Matantseva, Olga; Rollog, Mark; Kalvelage, Tim; Klockgether, Gabriele; Lavik, Gaute; Jetten, Mike S M; Kartal, Boran; Kuypers, Marcel M M  Nitrogen isotope effects induced by anammox bacteria  0027-8424  Proceedings of the National Academy of Sciences of the United States of America  Publication: JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift) 
2392731  Wenk, Christine B.; Zopfi, Jakob; Blees, Jan; Veronesi, Mauro; Niemann, Helge; Lehmann, Moritz F.  Community N and O isotope fractionation by sulfide-dependent denitrification and anammox in a stratified lacustrine water column  0016-7037  Geochimica et cosmochimica acta  Publication: JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift) 
   

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