A monoclonal antibody specific for snRNPs U1 and U2
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
ID 59466
Author(s) Reuter, R; Lehner, C F; Nigg, E A; Lührmann, R
Author(s) at UniBasel Nigg, Erich
Year 1986
Title A monoclonal antibody specific for snRNPs U1 and U2
Journal FEBS letters
Volume 201
Number 1
Pages / Article-Number 25-30
Abstract A monoclonal antibody (D-5) is described which selectively precipitates snRNPs U1 and U2. The antibody was derived from a mouse immunized with extracts from chick embryonic nuclei. By immunoblotting on either total proteins from purified snRNPs U1-U6, U2-U6 or U1 only, we could demonstrate that the monoclonal antibody cross-reacts with the U1 RNP specific polypeptide A and the U2 RNP specific polypeptide B", thereby establishing that the two snRNP proteins share at least one epitope. D-5 precipitates snRNPs U1 and U2 from a variety of species, including man, chicken, mouse, rat kangaroo and Xenopus laevis. It will thus be a useful tool for studying structure function relationships of the two snRNP species in different cell systems.
Publisher Elsevier Science
ISSN/ISBN 0014-5793
edoc-URL http://edoc.unibas.ch/dok/A5249516
Full Text on edoc No
Digital Object Identifier DOI 10.1016/0014-5793(86)80564-6
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/2423369
ISI-Number WOS:A1986C759100006
Document type (ISI) Journal Article

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