Active cyclin B1-Cdk1 first appears on centrosomes in prophase
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 59341
Author(s) Jackman, Mark; Lindon, Catherine; Nigg, Erich A; Pines, Jonathon
Author(s) at UniBasel Nigg, Erich
Year 2003
Title Active cyclin B1-Cdk1 first appears on centrosomes in prophase
Journal Nature Cell Biology
Volume 5
Number 2
Pages / Article-Number 143-8
Abstract Cyclin B1-Cdk1 is the key initiator of mitosis, but when and where activation occurs has not been precisely determined in mammalian cells. Activation may occur in the nucleus or cytoplasm, as just before nuclear envelope breakdown, Polo-like kinase1 (Plk1) is proposed to phosphorylate cyclin B1 in its nuclear export sequence (NES), to trigger rapid nuclear import. We raised phospho-specific antibodies against cyclin B1 that primarily recognise the active form of the complex. We show that cyclin B1 is initially phosphorylated on centrosomes in prophase and that Plk1 phosphorylates cyclin B1, but not in the NES. Furthermore, phosphorylation by Plk1 does not cause cyclin B1 to move into the nucleus. We conclude that cyclin B1-Cdk1 is first activated in the cytoplasm and that centrosomes may function as sites of integration for the proteins that trigger mitosis.
Publisher MacMillan
ISSN/ISBN 1465-7392
edoc-URL http://edoc.unibas.ch/dok/A5249397
Full Text on edoc No
Digital Object Identifier DOI 10.1038/ncb918
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/12524548
ISI-Number WOS:000180802100014
Document type (ISI) Journal Article
 
   

MCSS v5.8 PRO. 0.764 sec, queries - 0.000 sec ©Universität Basel  |  Impressum   |    
15/08/2020