A complex of two centrosomal proteins, CAP350 and FOP, cooperates with EB1 in microtubule anchoring
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 59304
Author(s) Yan, Xiumin; Habedanck, Robert; Nigg, Erich A.
Author(s) at UniBasel Nigg, Erich
Year 2006
Title A complex of two centrosomal proteins, CAP350 and FOP, cooperates with EB1 in microtubule anchoring
Journal Molecular Biology of the Cell
Volume 17
Number 2
Pages / Article-Number 634-644
Abstract The anchoring of microtubules (MTs) to subcellular structures is critical for cell shape, polarity, and motility. In mammalian cells, the centrosome is a prominent MT anchoring structure. A number of proteins, including ninein, p150Glued, and EB1, have been implicated in centrosomal MT anchoring, but the process is far from understood. Here we show that CAP350 and FOP (FGFR1 oncogene partner) form a centrosomal complex required for MT anchoring. We show that the C-terminal domain of CAP350 interacts directly with FOP and that both proteins localize to the centrosome throughout the cell cycle. FOP also binds to EB1 and is required for localizing EB1 to the centrosome. Depletion of either CAP350, FOP, or EB1 by siRNA causes loss of MT anchoring and profound disorganization of the MT network. These results have implications for the mechanisms underlying MT anchoring at the centrosome and they attribute a key MT anchoring function to two novel centrosomal proteins, CAP350 and FOP.
Publisher American Society for Cell Biology
ISSN/ISBN 1059-1524 ; 1939-4586
edoc-URL http://edoc.unibas.ch/dok/A5249362
Full Text on edoc Available
Digital Object Identifier DOI 10.1091/mbc.E05-08-0810
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/16314388
ISI-Number WOS:000235117300008
Document type (ISI) Article
 
   

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