Application of a Brown Trout (Salmo trutta fario) Ex Vivo Gonad Culture for Investigating the Effects of Chemical Exposure on Steroidogenesis
Third-party funded project
Project title Application of a Brown Trout (Salmo trutta fario) Ex Vivo Gonad Culture for Investigating the Effects of Chemical Exposure on Steroidogenesis
Principal Investigator(s) Holm, Patricia
Co-Investigator(s) Thorpe, Karen
A Marca-Pereira, Maria
Project Members Holm, Patricia
Organisation / Research unit Departement Umweltwissenschaften / Aquatische Ökologie (Holm)
Project start 01.06.2009
Probable end 31.03.2010
Status Completed
Abstract

The aim of this project is to develop a cost-effective in vitro assay of brown trout gonads to quantify the molecular and cellular effects of aromatase inhibitors (AIs). AIs are chemicals that affect reproduction through inhibiting the action of the aromatase enzyme that catalyses the final conversion of androgens into estrogens.To encompass the sensitive period of gonadal development, the gonads will be collected from immature males and females from each species. Primary endpoints for the assay will include the synthesis of sex steroids (testosterone, estradiol) to assess the function of the sex steroidogenic pathways,  histology to assess morphology and immunohistochemistry to assess cell proliferation

Keywords aromatase inhibitor, sex steroid, fish, brown trout, in-vitro assay, gonad culture
Financed by Foundations and Associations
   

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01/10/2023