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An evaluation of urine-CCA strip test and fingerprick blood SEA-ELISA for detection of urinary schistosomiasis in schoolchildren in Zanzibar
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
ID
533207
Author(s)
Stothard, J. R.; Sousa-Figueiredo J. C.,; Standley, C.; Van Dam G. J.,; Knopp, S.; Utzinger, J.; Ameri, H.; Khamis, A. N.; Khamis, I. S.; Deelder, A. M.; Mohammed, K. A.; Rollinson, D.
An evaluation of urine-CCA strip test and fingerprick blood SEA-ELISA for detection of urinary schistosomiasis in schoolchildren in Zanzibar
Journal
Acta tropica : Zeitschrift für Tropenwissenschaften und Tropenmedizin = revue des sciences tropicales et de médecine tropicale = review of tropical science and tropical medicine
To develop better monitoring protocols for detection of urinary schistosomiasis during ongoing control interventions, two commercially available diagnostic tests - the urine-circulating cathodic antigen (CCA) strip and the soluble egg antigen enzyme-linked immunosorbent assay (SEA-ELISA) - were evaluated for detection of Schistosoma haematobium infections in 150 schoolchildren from Zanzibar. The children originated from five primary schools representative of different levels of disease endemicity across the island; using standard urine filtration assessment with microscopy, mean prevalence of S. haematobium was 30.7% (95% confidence interval (CI)=23.4-38.7%) and a total of 35.3% (95% CI=27.7-43.5%) and 8.0% (95% CI=4.2-13.6%) children presented with micro- and macro-haematuria, respectively. Diagnostic scores of the urine-CCA strip were not satisfactory, a very poor sensitivity of 9% (95% CI=2-21%) was observed, precluding any further consideration. By contrast, the performance of the SEA-ELISA using sera from fingerprick blood was good; a sensitivity of 89% (95% CI=76-96%), a specificity of 70% (95% CI=60-79%), a positive predictive value of 57% (95% CI=45-69%) and a negative predictive value of 90% (95% CI=86-98%) were found. At the unit of the school, a positive linear association between prevalence inferred from parasitological examination and SEA-ELISA methods was found. The SEA-ELISA holds promise as a complementary field-based method for monitoring infection dynamics in schoolchildren over and above standard parasitological methods
