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Foot mucus and periostracum fraction as non-destructive source of DNA in the land snail Arianta arbustorum, and the development of new microsatellite loci
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
Foot mucus and periostracum fraction as non-destructive source of DNA in the land snail Arianta arbustorum, and the development of new microsatellite loci
Journal
Conservation genetics
Volume
6
Number
2
Pages / Article-Number
313-316
Keywords
DNA isolation, fingerprinting, gastropods, non-invasive genetics
Abstract
Feathers, hair, egg membranes, bones, urine and faeces have all been utilized as non-invasive sources of DNA in vertebrate studies. In insects, exuviae and caterpillar frass can serve as non-destructive DNA source. Similar non-invasive sources of DNA are not yet available for endangered and rare molluscs. In this note, we report on two novel, nondestructive methods of DNA isolation in the land snail Arianta arbustorum (L.), and present nine novel microsatellite loci since previously published loci yielded a complete lack of heterozygosity.