3D reconstruction from 2D crystal image and diffraction data
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
ID 494547
Author(s) Schenk, Andreas D; Castaño-Díez, Daniel; Gipson, Bryant; Arheit, Marcel; Zeng, Xiangyan; Stahlberg, Henning
Author(s) at UniBasel Stahlberg, Henning
Castano Diez, Daniel
Gipson, Bryant
Arheit, Marcel
Year 2010
Title 3D reconstruction from 2D crystal image and diffraction data
Journal Methods in enzymology
Volume 482
Pages / Article-Number 101-29

Electron crystallography of 2D protein crystals can determine the structure of membrane embedded proteins at high resolution. Images or electron diffraction patterns are recorded with the electron microscope of the frozen hydrated samples, and the 3D structure of the proteins is then determined by computer data processing. Here we introduce the image-processing algorithms for crystallographic Fourier space based methods using the Medical Research Council (MRC) programs, and illustrate the usage of the software packages 2dx, XDP, and IPLT.

Publisher Elsevier
ISSN/ISBN 0076-6879
edoc-URL http://edoc.unibas.ch/dok/A5842539
Full Text on edoc No
Digital Object Identifier DOI 10.1016/S0076-6879(10)82004-X
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/20888959
ISI-Number WOS:000283462200004
Document type (ISI) Journal Article

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