A single point mutation in the novel PvCesA3 gene confers resistance to the carboxylic acid amide fungicide mandipropamid in Plasmopara viticola
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 489679
Author(s) Blum, Mathias; Waldner, Maya; Gisi, Ulrich
Author(s) at UniBasel Blum, Mathias
Gisi, Ulrich
Year 2010
Title A single point mutation in the novel PvCesA3 gene confers resistance to the carboxylic acid amide fungicide mandipropamid in Plasmopara viticola
Journal Fungal Genetics and Biology
Volume 47
Number 6
Pages / Article-Number 499-510
Keywords CesA genes, Cellulose synthases, Fungicide resistance, Grapevine downy mildew, Inheritance, Single nucleotide polymorphism (SNP)
Abstract

The grapevine downy mildew, Plasmopara viticola, is one of the most devastating pathogens in viticulture. Effective control is mainly based on fungicide treatments, although resistance development in this pathogen is reported for a number of fungicides. In this study we describe for the first time the molecular mechanism of resistance to a carboxylic acid amide (CAA) fungicide. We identified a family of four cellulose synthase (CesA) genes containing conserved domains that are found in all processive glycosyltransferases. Phylogenetic analysis revealed their close relationship to the cellulose synthases of Phytophthora sp. Sequencing of the CesA genes in a CAA- resistant and -sensitive field isolate revealed five single nucleotide polymorphisms (SNPs) affecting the amino acid structure of the proteins. SNP inheritance in F-1-, F-2- and F-3-progeny confirmed resistance to be correlated with one single SNP located in PvCesA3. Only if present in both alleles, this SNP led to the substitution of a glycine for a serine residue at position 1105 (G1105S) in the deduced amino acid sequence, thus conferring CAA- resistance. Our data demonstrate that the identified genes are putative cellulose synthases and that one recessive mutation in PvCesA3 causes inheritable resistance to the CAA fungicide mandipropamid. (C) 2010 Elsevier Inc. All rights reserved.

Publisher Elsevier
ISSN/ISBN 1087-1845
URL http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WFV-4YK2F8S-1&_user=946149&_coverDate=06%2F30%2F2010&_rdoc=1&_fmt=high&_orig=search&_origin=search&_sort=d&_docanchor=&view=c&_acct=C000049002&_version=1&_urlVersion=0&_userid=946149&md5=850d3404d960269f27fd1982caa2f431&searchtype=a
edoc-URL http://edoc.unibas.ch/dok/A5842383
Full Text on edoc No
Digital Object Identifier DOI 10.1016/j.fgb.2010.02.009
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/20226261
ISI-Number WOS:000278170800001
Document type (ISI) Journal Article
 
   

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