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The Arabidopsis bZIP transcription factor HY5 regulates expression of the; PFG1/MYB12; gene in response to light and ultraviolet-B radiation
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 487855
Author(s) Stracke, Ralf; Favory, Jean-Jacques; Gruber, Henriette; Bartelniewoehner, Lutz; Bartels, Sebastian; Binkert, Melanie; Funk, Markus; Weisshaar, Bernd; Ulm, Roman
Author(s) at UniBasel Merker, Sebastian
Year 2010
Title The Arabidopsis bZIP transcription factor HY5 regulates expression of the; PFG1/MYB12; gene in response to light and ultraviolet-B radiation
Journal Plant, cell & environment
Volume 33
Number 1
Pages / Article-Number 88-103
Keywords abiotic stress, flavonoids, gene expression, MYB12, UV-B tolerance
Abstract

Plants fend off potentially damaging ultraviolet (UV)-B radiation by synthesizing and accumulating UV-B-absorbing flavonols that function as sunscreens. Regulation of this biosynthetic pathway is largely transcriptional and controlled by a network of transcription factors, among which the PRODUCTION OF FLAVONOL GLYCOSIDES (PFG) family of R2R3-MYB transcription factors was recently identified with a pivotal function. Here, we describe the response of Arabidopsis seedlings to narrow-band UV-B radiation at the level of phenylpropanoid pathway genes using whole-genome transcriptional profiling and identify the corresponding flavonol glycosides accumulating under UV-B. We further show that the bZIP transcriptional regulator ELONGATED HYPOCOTYL5 (HY5) is required for the transcriptional activation of the PFG1/MYB12 and PFG3/MYB111 genes under UV-B and visible light. A synthetic protein composed of HY5 with the VP16 activation domain is sufficient to activate PFG1/MYB12 expression in planta. However, even though myb11 myb12 myb111 triple mutants have strongly reduced CHS levels in darkness as well as in constant light, neither light- nor UV-B-inducibility seems impaired. Notwithstanding this, absence of the three PFG family transcription factors results in reduced UV-B tolerance, whereas PFG1/MYB12 overexpression leads to an increased tolerance. Thus, our data suggest that HY5-dependent regulation of PFG gene expression contributes to the establishment of UV-B tolerance.

Publisher Blackwell
ISSN/ISBN 0140-7791
edoc-URL http://edoc.unibas.ch/dok/A5842307
Full Text on edoc No
Digital Object Identifier DOI 10.1111/j.1365-3040.2009.02061.x
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/19895401
ISI-Number ISI:000272661000008
Document type (ISI) Journal Article
 
   

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