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A novel approach to single-cell analysis reveals intrinsic differences in immune marker expression in unstimulated BALB/c and C57BL/6 macrophages
JournalItem (Reviews, Editorials, Rezensionen, Urteilsanmerkungen etc. in einer wissenschaftlichen Zeitschrift)
 
ID 4650312
Author(s) Breda, Jeremie; Banerjee, Arka; Jayachandran, Rajesh; Pieters, Jean; Zavolan, Mihaela
Author(s) at UniBasel Zavolan, Mihaela
Breda, Jeremie
Banerjee, Arka
Jayachandran, Rajesh
Pieters, Jean
Year 2022
Title A novel approach to single-cell analysis reveals intrinsic differences in immune marker expression in unstimulated BALB/c and C57BL/6 macrophages
Journal FEBS Letters
Volume 596
Number 20
Pages 2630-2643
Keywords BALB/c mice, C57BL/6 mice, M1/M2 markers, macrophage heterogeneity, scRNA-seq
Mesh terms Mice; Animals; Mice, Inbred C57BL; Single-Cell Analysis; Mice, Inbred BALB C; Macrophages, metabolism; Biomarkers, metabolism
Abstract The origin of functional heterogeneity among macrophages, key innate immune system components, is still debated. While mouse strains differ in their immune responses, the range of gene expression variation among their pre-stimulation macrophages is unknown. With a novel approach to scRNA-seq analysis, we reveal the gene expression variation in unstimulated macrophage populations from BALB/c and C57BL/6 mice. We show that intrinsic strain-to-strain differences are detectable before stimulation and we place the unstimulated single cells within the gene expression landscape of stimulated macrophages. C57BL/6 mice show stronger evidence of macrophage polarization than BALB/c mice, which may contribute to their relative resistance to pathogens. Our computational methods can be generally adopted to uncover biological variation between cell populations.
Publisher Elsevier
ISSN/ISBN 0014-5793 ; 1873-3468
edoc-URL https://edoc.unibas.ch/90088/
Full Text on edoc Available
Digital Object Identifier DOI 10.1002/1873-3468.14478
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/36001069
Document type (ISI) Journal Article
 
   

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