Untargeted sequencing of circulating microRNAs in a healthy and diseased older population
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
ID 4643032
Author(s) Streese, Lukas; Demougin, Philippe; Iborra, Paula; Kanitz, Alexander; Deiseroth, Arne; Kröpfl, Julia M.; Schmidt-Trucksäss, Arno; Zavolan, Mihaela; Hanssen, Henner
Author(s) at UniBasel Zavolan, Mihaela
Streese, Lukas
Demougin, Philippe
Iborra de Toledo, Paula
Kanitz, Alexander
Deiseroth, Arne
Kröpfl, Julia Maria
Schmidt-Trucksäss, Arno
Hanssen, Henner
Year 2022
Title Untargeted sequencing of circulating microRNAs in a healthy and diseased older population
Journal Scientific Reports
Volume 12
Number 1
Pages / Article-Number 2991
Mesh terms Adaptation, Physiological, genetics; Age Factors; Cardiorespiratory Fitness; Circulating MicroRNA, genetics, metabolism, physiology; Cohort Studies; Disease, genetics; Exercise, genetics; Female; Gene Expression, genetics; Gene Expression Profiling, methods; Healthy Volunteers; Heart Disease Risk Factors; Humans; Male; Sedentary Behavior
Abstract We performed untargeted profiling of circulating microRNAs (miRNAs) in a well characterized cohort of older adults to verify associations of health and disease-related biomarkers with systemic miRNA expression. Differential expression analysis revealed 30 miRNAs that significantly differed between healthy active, healthy sedentary and sedentary cardiovascular risk patients. Increased expression of miRNAs miR-193b-5p, miR-122-5p, miR-885-3p, miR-193a-5p, miR-34a-5p, miR-505-3p, miR-194-5p, miR-27b-3p, miR-885-5p, miR-23b-5b, miR-365a-3p, miR-365b-3p, miR-22-5p was associated with a higher metabolic risk profile, unfavourable macro- and microvascular health, lower physical activity (PA) as well as cardiorespiratory fitness (CRF) levels. Increased expression of miR-342-3p, miR-1-3p, miR-92b-5p, miR-454-3p, miR-190a-5p and miR-375-3p was associated with a lower metabolic risk profile, favourable macro- and microvascular health as well as higher PA and CRF. Of note, the first two principal components explained as much as 20% and 11% of the data variance. miRNAs and their potential target genes appear to mediate disease- and health-related physiological and pathophysiological adaptations that need to be validated and supported by further downstream analysis in future studies.Clinical Trial Registration: ClinicalTrials.gov: NCT02796976 ( https://clinicaltrials.gov/ct2/show/NCT02796976 ).
Publisher Nature Publishing Group
ISSN/ISBN 2045-2322
edoc-URL https://edoc.unibas.ch/88159/
Full Text on edoc Available
Digital Object Identifier DOI 10.1038/s41598-022-06956-4
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/35194110
ISI-Number WOS:000759999200031
Document type (ISI) Journal Article

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