Impact of the clinically approved Petasites hybridus extract Ze 339 on intestinal mechanisms involved in the handling of histamine.
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 4642928
Author(s) Mettler, Lina G; Brecht, Karin; Butterweck, Veronika; Meyer Zu Schwabedissen, Henriette E
Author(s) at UniBasel Meyer zu Schwabedissen, Henriette
Brecht Brüngger, Karin
Mettler, Lina Giulietta
Year 2022
Title Impact of the clinically approved Petasites hybridus extract Ze 339 on intestinal mechanisms involved in the handling of histamine.
Journal Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
Volume 148
Pages / Article-Number 112698
Keywords Diamine oxidase; Histamine intolerance; Histamine transport; Intestinal histamine handling; OCT3 (SLC22A3); Petasites hybridus
Abstract

In patients with histamine intolerance accumulated or ingested histamine causes a broad range of undesirable symptoms. Food-derived histamine is degraded by intestinal diamine oxidase (DAO) and histamine-N-methyltransferase (HNMT), while the organic cation transporter 3 (OCT3) contributes to the transcellular flux of histamine. Anecdotal evidence from patients with HIT suggests an improvement of symptoms related to histamine intolerance after intake of Ze 339, a lipophilic CO; 2; -extract prepared from the leaves of Petasites hybridus. Thus, it was the aim of this study to investigate the influence of Ze 339 on DAO, HNMT and OCT3 using Caco-2 and MDCKII cells. Even though Ze 339 reduced mRNA levels of HNMT and DAO, there was no change in protein expression. Ze 339 changed neither the basal release nor the enzymatic activity of DAO. Testing the interaction of Ze 339 with the transcellular histamine transport, we observed a significant increase in the basal to apical flux in presence of high Ze 339 concentrations at the early phases of the experiment. Testing the influence of Ze 339 on OCT3-mediated histamine uptake in overexpressing MDCKII cells revealed a dose-dependent inhibition with an estimated IC; 50; of 26.9 ug/mL for the extract. In conclusion, we report an effect of Ze 339 on transcellular histamine transport, where inhibition of OCT3 may contribute.

ISSN/ISBN 1950-6007
Full Text on edoc
Digital Object Identifier DOI 10.1016/j.biopha.2022.112698
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/35149385
   

MCSS v5.8 PRO. 0.317 sec, queries - 0.000 sec ©Universität Basel  |  Impressum   |    
10/08/2022