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Elucidating Drought-Tolerance Mechanisms in Plant Roots through 1H NMR Metabolomics in Parallel with MALDI-MS, and NanoSIMS Imaging Techniques
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
ID 4641522
Author(s) Honeker, Linnea K.; Hildebrand, Gina A.; Fudyma, Jane D.; Daber, L. Erik; Hoyt, David; Flowers, Sarah E.; Gil-Loaiza, Juliana; Kübert, Angelika; Bamberger, Ines; Anderton, Christopher R.; Cliff, John; Leichty, Sarah; Amini Tabrizi, Roya; Kreuzwieser, Jürgen; Shi, Lingling; Bai, Xuejuan; Velickovic, Dusan; Dippold, Michaela A.; Ladd, S. Nemiah; Werner, Christiane; Meredith, Laura K.; Tfaily, Malak M.
Author(s) at UniBasel Ladd, Sarah Nemiah
Year 2022
Title Elucidating Drought-Tolerance Mechanisms in Plant Roots through 1H NMR Metabolomics in Parallel with MALDI-MS, and NanoSIMS Imaging Techniques
Journal Environmental science and technology
Volume 56
Number 3
Pages / Article-Number 2021-2032
Keywords drought, carbon cycle, abiotic plant stress, position-specific pyruvate labeling, tropical rainforest
Abstract As direct mediators between plants and soil, roots play an important role in metabolic responses to environmental stresses such as drought, yet these responses are vastly uncharacterized on a plant-specific level, especially for co-occurring species. Here, we aim to examine the effects of drought on root metabolic profiles and carbon allocation pathways of three tropical rainforest species by combining cutting-edge metabolomic and imaging technologies in an in situ position-specific 13 C-pyruvate root-labeling experiment. Further, washed (rhizosphere-depleted) and unwashed roots were examined to test the impact of microbial presence on root metabolic pathways. Drought had a species-specific impact on the metabolic profiles and spatial distribution in Piper sp. and Hibiscus rosa sinensis roots, signifying different defense mechanisms; Piper sp. enhanced root structural defense via recalcitrant compounds including lignin, while H. rosa sinensis enhanced biochemical defense via secretion of antioxidants and fatty acids. In contrast, Clitoria fairchildiana , a legume tree, was not influenced as much by drought but rather by rhizosphere presence where carbohydrate storage was enhanced, indicating a close association with symbiotic microbes. This study demonstrates how multiple techniques can be combined to identify how plants cope with drought through different drought-tolerance strategies and the consequences of such changes on below-ground organic matter composition.
ISSN/ISBN 0194-0287
Full Text on edoc No
Digital Object Identifier DOI 10.1021/acs.est.1c06772
PubMed ID
ISI-Number WOS:000746494000001
Document type (ISI) Journal Article

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