Quantitative mRNA expression analysis of membrane bound uptake and efflux transporters in rodent tissues
Thesis (Dissertationen, Habilitationen)
 
ID 4627884
Author Vonwyl, Celina
Author at UniBasel Meyer zu Schwabedissen, Henriette
Year 2019
Title Quantitative mRNA expression analysis of membrane bound uptake and efflux transporters in rodent tissues
Type of Thesis Masterarbeit
Start of thesis 07.01.2019
End of thesis 31.05.2019
Name of University University of Basel
Name of Faculty Philosophisch-Naturwissenschaftliche Fakultät
Supervisor(s) / Fachvertreter/in Meyer zu Schwabedissen, Henriette
Abstract

This study focussed on the interspecies differences between the membrane transporter Organic
Anion Transporting Polypeptide 2B1 (OATP2B1) expressed in humans and rodents. In recent
years the relevance of this uptake transporter in the context of drug-drug- and food-drug
interaction gained attention. Since, OATP2B1 is ubiquitously expressed and many endogenous
and exogenous compounds are described as substrates, the uptake transporter may have an
essential role in pharmacokinetics. However, little is known about the orthologue in rats.
Former studies indicated similar expression patterns for the transport protein in human and rat,
but revealed differences in substrate specificity – namely steroid sulfates like estrone-3-sulfate
and dehydroepiandrosterone sulfate. Whereas the human orthologue seems to have a crucial
role in steroid metabolism, these steroids were not transported by rat Oatp2b1 (rOatp) indicating
different functions. With regard to a follow-up study, where the function of the human
OATP2B1 (hOATP2B1) will be studied in a humanized rat model, a method for absolute
quantification of transport proteins involved in uptake or efflux using SYBRTM Green
quantitative real-time polymerase chain (real-time qPCR) reaction was evaluated. Therefore
standard curves were generated and the mRNA expression of different transporters was
quantified using the respective standard curve for each transporter. To further characterize the
function of rOatp2b1 in vitro experiments with the model substrate atorvastatin were conducted.
These experiments showed that atorvastatin was transported by the rOatp2b1 even with a much
lower affinity as it is reported for the human orthologue. In addition, the atorvastatin uptake
was studied in the presence of eight different compounds previously reported as hOATP2B1
substrates or inhibitors. The intracellular atorvastatin concentration was significantly inhibition
by celiprolol and BSP.

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