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Bacterial Proteins, chemistry, metabolism; Biofilms, growth & development; Models, Molecular; Polysaccharides, Bacterial, metabolism; Protein Binding; Protein Conformation; Vibrio cholerae, physiology
Abstract
Biofilm formation is critical for the infection cycle of; Vibrio cholerae. Vibrio; exopolysaccharides (VPS) and the matrix proteins RbmA, Bap1 and RbmC are required for the development of biofilm architecture. We demonstrate that RbmA binds VPS directly and uses a binary structural switch within its first fibronectin type III (FnIII-1) domain to control RbmA structural dynamics and the formation of VPS-dependent higher-order structures. The structural switch in FnIII-1 regulates interactions in trans with the FnIII-2 domain, leading to open (monomeric) or closed (dimeric) interfaces. The ability of RbmA to switch between open and closed states is important for; V. cholerae; biofilm formation, as RbmA variants with switches that are locked in either of the two states lead to biofilms with altered architecture and structural integrity.
