Data Entry: Please note that the research database will be replaced by UNIverse by the end of October 2023. Please enter your data into the system https://universe-intern.unibas.ch. Thanks

Login for users with Unibas email account...

Login for registered users without Unibas email account...

 
Contribution of Hydrogen Cyanide to the Antagonistic Activity of Pseudomonas Strains Against Phytophthora infestans
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 4614910
Author(s) Anand, Abhishek; Chinchilla, Delphine; Tan, Christopher; Mène-Saffrané, Laurent; L'Haridon, Floriane; Weisskopf, Laure
Author(s) at UniBasel Chinchilla, Delphine
Year 2020
Title Contribution of Hydrogen Cyanide to the Antagonistic Activity of Pseudomonas Strains Against Phytophthora infestans
Journal Microorganisms
Volume 8
Number 8
Pages / Article-Number 1144
Keywords biocontrol, hydrogen cyanide, potato late blight, pseudomonads
Abstract Plants face many biotic and abiotic challenges in nature; one of them is attack by disease-causing microbes. Phytophthora infestans , the causal agent of late blight is one of the most prominent pathogens of the potato responsible for multi-billion-dollar losses every year. We have previously reported that potato-associated Pseudomonas strains inhibited P. infestans at various developmental stages. A comparative genomics approach identified several factors putatively involved in this anti-oomycete activity, among which was the production of hydrogen cyanide (HCN). Here, we report the relative contribution of HCN emission to the overall anti- Phytophthora activity of two cyanogenic Pseudomonas strains, P. putida R32 and P. chlororaphis R47. To quantify this contribution, we generated HCN-negative mutants (Δhcn) and compared their activities to those of their respective wild types in different experiments assessing P. infestans mycelial growth, zoospore germination, and infection of potato leaf disks. Using in vitro experiments allowing only volatile-mediated interactions, we observed that HCN accounted for most of the mycelial growth inhibition (57% in R47 and 80% in R32). However, when allowing both volatile and diffusible compound-mediated interactions, HCN only accounted for 1% (R47) and 18% (R32) of mycelial growth inhibition. Likewise, both mutants inhibited zoospore germination in a similar way as their respective wild types. More importantly, leaf disk experiments showed that both wild-type and Δhcn strains of R47 and R32 were able to limit P. infestans infection to a similar extent. Our results suggest that while HCN is a major contributor to the in vitro volatile-mediated restriction of P. infestans mycelial growth, it does not play a major role in the inhibition of other disease-related features such as zoospore germination or infection of plant tissues.
Publisher MDPI
ISSN/ISBN 2076-2607
URL http://www.ncbi.nlm.nih.gov/pmc/articles/pmc7464445/
edoc-URL https://edoc.unibas.ch/82558/
Full Text on edoc No
Digital Object Identifier DOI 10.3390/microorganisms8081144
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/32731625
Document type (ISI) Journal Article
 
   

MCSS v5.8 PRO. 0.324 sec, queries - 0.000 sec ©Universität Basel  |  Impressum   |    
29/03/2024