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Functional and Structural Insights into a Novel Promiscuous Ketoreductase of the Lugdunomycin Biosynthetic Pathway
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
ID
4612415
Author(s)
Xiao, Xiansha; Elsayed, Somayah S.; Wu, Changsheng; van der Heul, Helga U.; Metsä-Ketelä, Mikko; Du, Chao; Prota, Andrea E.; Chen, Chun-Chi; Liu, Weidong; Guo, Rey-Ting; Abrahams, Jan Pieter; van Wezel, Gilles P.
Functional and Structural Insights into a Novel Promiscuous Ketoreductase of the Lugdunomycin Biosynthetic Pathway
Journal
ACS Chemical Biology
Volume
15
Number
9
Pages / Article-Number
2529-2538
Abstract
Angucyclines are a structurally diverse class of actinobacterial natural products defined by their varied polycyclic ring systems, which display a wide range of biological activities. We recently discovered lugdunomycin (; 1; ), a highly rearranged polyketide antibiotic derived from the angucycline backbone that is synthesized via several yet unexplained enzymatic reactions. Here, we show via; in vivo; ,; in vitro; , and structural analysis that the promiscuous reductase LugOII catalyzes both a C6 and an unprecedented C1 ketoreduction. This then sets the stage for the subsequent C-ring cleavage that is key to the rearranged scaffolds of; 1; . The 1.1 Å structures of LugOII in complex with either ligand 8-; O; -Methylrabelomycin (; 4; ) or 8-; O; -Methyltetrangomycin (; 5; ) and of apoenzyme were resolved, which revealed a canonical Rossman fold and a remarkable conformational change during substrate capture and release. Mutational analysis uncovered key residues for substrate access, position, and catalysis as well as specific determinants that control its dual functionality. The insights obtained in this work hold promise for the discovery and engineering of other promiscuous reductases that may be harnessed for the generation of novel biocatalysts for chemoenzymatic applications.
