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Low-cost automated capillary electrophoresis instrument assembled from commercially available parts
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 4612228
Author(s) Furter, Jasmine S.; Boillat, Marc-Aurele; Hauser, Peter C.
Author(s) at UniBasel Hauser, Peter C.
Year 2020
Title Low-cost automated capillary electrophoresis instrument assembled from commercially available parts
Journal Electrophoresis
Volume 41
Number 24
Pages / Article-Number 2075-2082
Keywords Arduino; Capillary electrophoresis; Conductivity detection; Microfluidic breadboard approach; Microfluidics; Open source hardware
Mesh terms Science & TechnologyLife Sciences & BiomedicinePhysical SciencesBiochemical Research MethodsChemistry, AnalyticalBiochemistry & Molecular BiologyChemistry
Abstract A CE instrument that can be assembled from commercially available components with minimal construction effort is described. Except for the electronic control circuitry no specially made parts are required. It is based on a flexible design of microfluidic, electropneumatic, and electronic sections and different configurations can easily be implemented. Automated injection into the capillary is performed hydrodynamically by the application of a pressure for a controlled length of time. The performance of the device was tested with a contactless conductivity detector by separating different metal ions. In addition, nine metal cations related to the quality of honey were separated in 2.3 min and four honey samples were analysed quantitatively to demonstrate the applicability of the method.
Publisher Wiley
ISSN/ISBN 0173-0835 ; 1522-2683
edoc-URL https://edoc.unibas.ch/80567/
Full Text on edoc No
Digital Object Identifier DOI 10.1002/elps.202000211
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/32895993
ISI-Number 000571189500001
Document type (ISI) Journal Article
 
   

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