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Purified compounds from marine organism sea pen induce apoptosis in human breast cancer cell MDA-MB-231 and cervical cancer cell HeLa
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 4604285
Author(s) Sharifi, Sharareh; Mostafavi, Pargol Ghavam; Tarasi, Roghayeh; Moradi, Ali Mashinchian; Givianrad, Mohammad Hadi; Moridi Farimani, Mahdi; Ebrahimi, Samad Nejad; Hamburger, Matthias; Niknejad, Hassan
Author(s) at UniBasel Hamburger, Matthias
Year 2020
Title Purified compounds from marine organism sea pen induce apoptosis in human breast cancer cell MDA-MB-231 and cervical cancer cell HeLa
Journal European Journal of Pharmacology
Volume 877
Pages / Article-Number 173075
Mesh terms Animals; Antineoplastic Agents, pharmacology; Apoptosis, drug effects; Breast Neoplasms, pathology; Cell Proliferation, drug effects; Cell Survival, drug effects; Cnidaria, chemistry; Female; HeLa Cells; Humans; Uterine Cervical Neoplasms, pathology
Abstract Marine organisms are an important source of chemical compounds which are appropriate for use as therapeutic agents. Among them, Sea pens produce valuable chemical compounds being used as anti-cancer drugs. The aim of this study was to investigate anti-cancer property of extracted and purified compounds from marine organism Sea pen and evaluate their effects on inducing of apoptosis. The extracts were prepared from dried colony of Virgularia gustaviana. The compounds (3β)-Cholest,5en,3ol (cholesterol) (15 mg), Hexadecanoic acid (2.5 mg) and 2-Hexadecanol (10.7 mg) were identified by GC-MS and NMR. The cytotoxic effects of the compounds were evaluated on Hela and MDA-Mb-231 human cancer cell lines with MTT assay. Immunocytochemistry and Western Blot analyses were used to evaluate the expression of apoptosis related markers Caspase 3, Caspase 8, Bax and BCL2 in cancer cells after treating with three compounds. The purified compounds reduced viability of human breast cancer cell line MDA-MB-231 and human cervical cancer cell line Hela concentration-dependently. 2-Hexadecanol reduced significantly the viability of both cancer cell lines in comparison to the other purified compounds. Treatment of cancer cells with the three purified compounds increased the expression of caspase-3, caspase-8 and Bax proteins and decreased the relative Bcl-2/Bax ratio, demonstrating induction of apoptosis as possible mechanism of action. According to the results, three purified compounds inhibit the growth of cancer cells by inducing of apoptosis pathway; an effect which needs to be further investigated in the future studies.
Publisher Elsevier
ISSN/ISBN 0014-2999 ; 1879-0712
edoc-URL https://edoc.unibas.ch/78657/
Full Text on edoc No
Digital Object Identifier DOI 10.1016/j.ejphar.2020.173075
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/32222494
ISI-Number WOS:000532679600007
Document type (ISI) Journal Article
 
   

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