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Shedding Light on Metal-Based Nanoparticles in Zebrafish by Computed Tomography with Micrometer Resolution
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 4599241
Author(s) Cörek, Emre; Rodgers, Griffin; Siegrist, Stefan; Einfalt, Tomaz; Detampel, Pascal; Schlepütz, Christian M.; Sieber, Sandro; Fluder, Pascal; Schulz, Georg; Unterweger, Harald; Alexiou, Christoph; Müller, Bert; Puchkov, Maxim; Huwyler, Jörg
Author(s) at UniBasel Huwyler, Jörg
Çörek, Emre
Siegrist, Stefan
Einfalt, Tomaz
Detampel, Pascal
Sieber, Sandro
Müller, Bert
Rodgers, Griffin
Schulz, Georg
Puchkov, Maxim
Year 2020
Title Shedding Light on Metal-Based Nanoparticles in Zebrafish by Computed Tomography with Micrometer Resolution
Journal Small
Volume 16
Number 31
Pages / Article-Number e2000746
Keywords digital histology, nanoparticle detection, superparamagnetic iron oxide nanoparticles, synchrotron radiation‐based micro computed tomography, zebrafish embryos
Mesh terms Animals; Magnetite Nanoparticles; Metal Nanoparticles; Oxides; Tissue Distribution; Tomography, X-Ray Computed; Zebrafish
Abstract Metal‐based nanoparticles are clinically used for diagnostic and therapeutic applications. After parenteral administration, they will distribute throughout different organs. Quantification of their distribution within tissues in the 3D space, however, remains a challenge owing to the small particle diameter. In this study, synchrotron radiation‐based hard X‐ray tomography (SRμCT) in absorption and phase contrast modes is evaluated for the localization of superparamagnetic iron oxide nanoparticles (SPIONs) in soft tissues based on their electron density and X‐ray attenuation. Biodistribution of SPIONs is studied using zebrafish embryos as a vertebrate screening model. This label‐free approach gives rise to an isotropic, 3D, direct space visualization of the entire 2.5 mm‐long animal with a spatial resolution of around 2 µm. High resolution image stacks are available on a dedicated internet page ( http://zebrafish.pharma‐te.ch ). X‐ray tomography is combined with physico‐chemical characterization and cellular uptake studies to confirm the safety and effectiveness of protective SPION coatings. It is demonstrated that SRμCT provides unprecedented insights into the zebrafish embryo anatomy and tissue distribution of label‐free metal oxide nanoparticles.
Publisher Wiley
ISSN/ISBN 1613-6829
edoc-URL https://edoc.unibas.ch/77507/
Full Text on edoc No
Digital Object Identifier DOI 10.1002/smll.202000746
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/32567135
ISI-Number WOS:000541472100001
Document type (ISI) Journal Article
 
   

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