A simple, rapid typing method for Streptococcus agalactiae based on ribosomal subunit proteins by MALDI-TOF MS
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 4598463
Author(s) Rothen, Julian; Sapugahawatte, Dulmini Nanayakkara; Li, Carmen; Lo, Norman; Vogel, Guido; Foucault, Frédéric; Pflüger, Valentin; Pothier, Joёl F.; Blom, Jochen; Daubenberger, Claudia; Ip, Margaret
Author(s) at UniBasel Rothen, Julian
Daubenberger, Claudia
Year 2020
Title A simple, rapid typing method for Streptococcus agalactiae based on ribosomal subunit proteins by MALDI-TOF MS
Journal Scientific reports
Volume 10
Number 1
Pages / Article-Number 8788
Abstract Streptococcus agalactiae (Group B Streptococcus, GBS), is a frequent human colonizer and a leading cause of neonatal meningitis as well as an emerging pathogen in non-pregnant adults. GBS possesses a broad animal host spectrum, and recent studies proved atypical GBS genotypes can cause human invasive diseases through animal sources as food-borne zoonotic infections. We applied a MALDI-TOF MS typing method, based on molecular weight variations of predefined 28 ribosomal subunit proteins (rsp) to classify GBS strains of varying serotypes into major phylogenetic lineages. A total of 249 GBS isolates of representative and varying capsular serotypes from patients and animal food sources (fish and pig) collected during 2016-2018 in Hong Kong were analysed. Over 84% (143/171) noninvasive carriage GBS strains from patients were readily typed into 5 globally dominant rsp-profiles. Among GBS strains from food animals, over 90% (57/63) of fish and 13% (2/15) of pig GBS matched with existing rsp-profiles, while the remainder were classified into two novel rsp-profiles and we failed to assign a fish strain into any cluster. MALDI-TOF MS allowed for high-throughput screening and simultaneous detection of novel, so far not well described GBS genotypes. The method shown here is rapid, simple, readily transferable and adapted for use in a diagnostic microbiology laboratory with potential for the surveillance of emerging GBS genotypes with zoonotic potential.
Publisher Springer Nature
ISSN/ISBN 0169-5487
edoc-URL https://edoc.unibas.ch/76858/
Full Text on edoc Available
Digital Object Identifier DOI 10.1038/s41598-020-65707-5
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/32472028
ISI-Number MEDLINE:32472028
Document type (ISI) Journal Article
 
   

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11/08/2020