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Cre/loxP-based mouse models of mast cell deficiency and mast cell-specific gene inactivation
Book Item (Buchkapitel, Lexikonartikel, jur. Kommentierung, Beiträge in Sammelbänden) |
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ID |
4598197 |
Author(s) |
Peschke, Katrin; Dudeck, Anne; Rabenhorst, Anja; Hartmann, Karin; Roers, Axel |
Author(s) at UniBasel |
Hartmann, Karin
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Year |
2015 |
Title |
Cre/loxP-based mouse models of mast cell deficiency and mast cell-specific gene inactivation |
Editor(s) |
Hughes, Michael R.; McNagny, Kelly M. |
Book title |
Mast Cells: Methods and Protocols |
Publisher |
Humana Press |
Place of publication |
New York, NY |
Pages |
403-421 |
Mesh terms |
Alleles; Animals; Binding Sites; Chymases, genetics; Connective Tissue, immunology; DNA, genetics, isolation & purification; Flow Cytometry; Gene Deletion; Gene Knock-In Techniques; Gene Silencing; Genetic Engineering, methods; Genotyping Techniques; Integrases, metabolism; Mast Cells, cytology, metabolism; Mice; Polymerase Chain Reaction; Recombination, Genetic; Transgenes, genetics |
Abstract |
Over the past decades, research on in vivo functions of mast cells has largely relied on kit-mutant mouse strains. Recently, new mouse models for investigation of mast cell functions based on the Cre/loxP recombination system have been published and results in these new models challenged findings of previous studies in kit-mutant mice. Herein we describe procedures central to mast cell-specific gene inactivation and the generation of mast cell-deficient mice based on the mouse strain Mcpt5-Cre, which expresses Cre recombinase selectively in connective tissue mast cells. |
Full Text on edoc |
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Digital Object Identifier DOI |
10.1007/978-1-4939-1568-2_25 |
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23/04/2024
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