Efficient Dual-Negative Selection for Bacterial Genome Editing

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Efficient Dual-Negative Selection for Bacterial Genome Editing

Discussion paper / Internet publication

ID	4597330
Digital Object Identifier DOI	10.1101/2020.03.03.974816
Author(s)	Cianfanelli, Francesca Romana; Cunrath, Olivier; Bumann, Dirk
Author(s) at UniBasel	Bumann, Dirk
Year	2020
Month and day	03-04
Title	Efficient Dual-Negative Selection for Bacterial Genome Editing
Pages	16
Publisher / Institution	bioRxiv
URL	https://www.biorxiv.org/content/10.1101/2020.03.03.974816v1.full.pdf+html
Abstract	We describe a versatile method for chromosomal gene editing based on classical consecutive single-crossovers. The method exploits rapid plasmid construction using Gibson assembly, a convenient E. coli donor strain, and efficient dual-negative selection for improved suicide vector resolution. We used this method to generate in frame deletions, insertions and point mutations in Salmonella enterica with limited hands-on time. Similar strategies allowed efficient gene editing also in Pseudomonas aeruginosa and multi-drug-resistant (MDR) Escherichia coli clinical isolates.
edoc-URL	https://edoc.unibas.ch/76528/
Full Text on edoc	Restricted

02/05/2024

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