In Vitro drug efficacy testing against Trypanosoma brucei

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In Vitro drug efficacy testing against Trypanosoma brucei

Edited Book (Herausgeber eines eigenständigen Buches)

ID	4596686
Editor(s)	Michels, P.A.M.; Ginger, M.L.; Zilberstein, D.
Editor(s) at UniBasel	Kaiser, Marcel Mäser, Pascal
Year	2020
Title	In Vitro drug efficacy testing against Trypanosoma brucei
Edition	Michels, PAM; Ginger, ML; Zilberstein, D
Publisher	Springer
Place of publication	New York
ISSN/ISBN	978-1-0716-0293-5
Volume	46
Abstract	The recent endorsement of fexinidazole by the European Medicines Agency for the treatment of human African trypanosomiasis has demonstrated the high predictive value of cell-based assays for parasite chemotherapy. Here we describe three in vitro drug susceptibility tests with Trypanosoma brucei that have served as the basis for the identification of fexinidazole as a promising lead: (1) a standard assay with end-point measurement to determine drug efficacy; (2) a wash-out assay to test for reversibility and speed of drug action; (3) isothermal microcalorimetry for real-time measurement of onset of drug action and time to kill. Together, these assays allow to estimate pharmacodynamic parameters in vitro and to devise appropriate treatment regimens for subsequent in vivo experiments.
edoc-URL	https://edoc.unibas.ch/76233/
Full Text on edoc	No
Digital Object Identifier DOI	10.1007/978-1-0716-0294-2_46

12/05/2024

Research Database / FORSCHUNGSDATENBANK