Data Entry: Please note that the research database will be replaced by UNIverse by the end of October 2023. Please enter your data into the system https://universe-intern.unibas.ch. Thanks

Login for users with Unibas email account...

Login for registered users without Unibas email account...

 
Microfluidic Sample Preparation for High-Resolution Electron Microscopy, Visual Proteomics and Electron Tomography
Third-party funded project
Project title Microfluidic Sample Preparation for High-Resolution Electron Microscopy, Visual Proteomics and Electron Tomography
Principal Investigator(s) Braun, Thomas
Co-Investigator(s) Pecho-Vrieseling, Eline
Organisation / Research unit Departement Biozentrum / Structural Biology (Braun),
Departement Biozentrum / Structural Biology (Stahlberg)
Project start 01.04.2020
Probable end 31.03.2024
Status Active
Abstract

Electron microscopy (EM) introduced a fast and lasting change to structural and cellular biology. Direct electron detector cameras and improved image processing algorithms now allow structure determination of large biomolecules by cryogenic EM (cryo-EM) at atomic resolution using a single particle approach.
Strategies to study the cellular ultrastructure, such as electron tomography (ET), correlative light and electron microscopy (CLEM), and the lamella milling of eukaryotic cells, opened new windows allowing biologists to study the mechanism of cellular processes at unprecedented precision.
Unfortunately, sample preparation remains a bottleneck, and, surprisingly, EM is rarely used as a bioanalytical tool despite its immense potential to detect proteins on the single-molecule level. Both (i) new single-cell analysis tools and (ii) advanced methods for protein isolation and cryo-EM sample preparation are urgently needed. Here, we aim at (i) the development of a versatile system for the fast protein production, protein isolation, and cryo-EM sample preparation for the structural analysis of sensitive protein complexes, (ii) the development of a new targeted and untargeted single-cell analysis method named "single-cell visual proteomics," and (iii) the development of a new strategy for the blotting-free cryopreservation of eukaryotic cells to study cellular structures by ET.

Financed by Swiss National Science Foundation (SNSF)
Follow-up project of 3242170 Fast protein-complex isolation, sample preparation and data processing for high-resolution structural analysis and visual proteomics
   

MCSS v5.8 PRO. 0.409 sec, queries - 0.000 sec ©Universität Basel  |  Impressum   |    
28/03/2024