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Methods used in the structure determination of bovine mitochondrial F-1 ATPase
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 4531105
Author(s) Abrahams, J. P.; Leslie, A. G. W.
Author(s) at UniBasel Abrahams, Jan Pieter
Year 1996
Title Methods used in the structure determination of bovine mitochondrial F-1 ATPase
Journal Acta Crystallographica. Section D, Biological Crystallography
Volume 52
Pages / Article-Number 30-42
Mesh terms Science & TechnologyLife Sciences & BiomedicinePhysical SciencesBiochemical Research MethodsBiochemistry & Molecular BiologyBiophysicsCrystallographyBiochemistry & Molecular BiologyBiophysicsCrystallography
Abstract With a size of 372 kDa, the F-1 ATPase particle is the largest asymmetric structure solved to date. Isomorphous differences arising from reacting the crystals with methyl-mercury nitrate at two concentrations allowed the structure determination, Careful data collection and data processing were essential in this process as well as a new form of electron-density modification, 'solvent flipping'. The most important feature of this new procedure is that the electron density in the solvent region is inverted rather than set to a constant value, as in conventional solvent flattening. All non-standard techniques and variations on new techniques which were employed in the structure determination are described.
Publisher Munksgaard
ISSN/ISBN 0907-4449
edoc-URL https://edoc.unibas.ch/76007/
Full Text on edoc No
Digital Object Identifier DOI 10.1107/S0907444995008754
ISI-Number 1996TT22600003
Document type (ISI) Article
 
   

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