1COW: Bovine Mitochondrial F1-Atpase Complexed With Aurovertin B
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
ID 4531101
Author(s) Van Raaij, M; Abrahams, JP; Leslie, AGW; Walker, JE
Author(s) at UniBasel Abrahams, Jan Pieter
Year 1996
Title 1COW: Bovine Mitochondrial F1-Atpase Complexed With Aurovertin B
Journal Worldwide Protein Data Bank
Pages / Article-Number 1COW
Keywords Hydrogen ion transport
Mesh terms Science & TechnologyLife Sciences & BiomedicineBiochemistry & Molecular BiologyBiochemistry & Molecular Biology

In the structure of bovine mitochondrial F1-ATPase that was previously determined with crystals grown in the presence of adenylyl-imidodiphosphate (AMP-PNP) and ADP, the three catalytic beta-subunits have different conformations and nucleotide occupancies. Adenylyl-imidodiphosphate is bound to one beta-subunit (betaTP), ADP is bound to the second (betaDP), and no nucleotide is bound to the third (betaE). Here we show that the uncompetitive inhibitor aurovertin B binds to bovine F1 at two equivalent sites in betaTP and betaE, in a cleft between the nucleotide binding and C-terminal domains. In betaDP, the aurovertin B pocket is incomplete and is inaccessible to the inhibitor. The aurovertin B bound to betaTP interacts with alpha-Glu399 in the adjacent alphaTP subunit, whereas the aurovertin B bound to betaE is too distant from alphaE to make an equivalent interaction. Both sites encompass betaArg-412, which was shown by mutational studies to be involved in binding aurovertin. Except for minor changes around the aurovertin pockets, the structure of bovine F1-ATPase is the same as determined previously. Aurovertin B appears to act by preventing closure of the catalytic interfaces, which is essential for a catalytic mechanism involving cyclic interconversion of catalytic sites.

Full Text on edoc
Digital Object Identifier DOI 10.2210/pdb1cow/pdb
ISI-Number DRCI:DATA2012135001714017
Document type (ISI) Data set

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