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Screening crystallisation conditions using fluorescence correlation spectroscopy
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 4531065
Author(s) Schmauder, R.; Schmidt, T.; Abrahams, J. P.; Kuil, M. E.
Author(s) at UniBasel Abrahams, Jan Pieter
Year 2002
Title Screening crystallisation conditions using fluorescence correlation spectroscopy
Journal Acta Crystallographica Section D: Structural Biology
Volume 58
Number Pt 10 Pt 1
Pages / Article-Number 1536-41
Keywords diffusion; lysozyme; fluorescence correlation spectroscopy; crystallisation conditions
Mesh terms Science & TechnologyLife Sciences & BiomedicinePhysical SciencesBiochemical Research MethodsBiochemistry & Molecular BiologyBiophysicsCrystallographyBiochemistry & Molecular BiologyBiophysicsCrystallography
Abstract We investigate the potential of fluorescence correlation spectroscopy (FCS) in screening for crystallisation conditions. Solutions that nucleate protein crystals must have different interactions than solutions that do not give rise to crystals. Due to these different interactions the average mean squared displacement of the individual proteins changes. By monitoring protein self-diffusion, we can distinguish crystallising from non-crystallising solutions. The method introduced can be applied at extremely low concentrations in femtoliter volumes as an early diagnostic for molecular association. Based on our preliminary findings FCS has the potential to become a routine screening method for crystallography.
Publisher International Union of Crystallography
ISSN/ISBN 2059-7983
edoc-URL https://edoc.unibas.ch/75980/
Full Text on edoc No
Digital Object Identifier DOI 10.1107/S0907444902014373
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/12351856
ISI-Number 000178248000007
Document type (ISI) Journal Article
 
   

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