3C0S: UVDE 3 metals
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 4531014
Author(s) Meulenbroek, EM; Paspaleva, K; Thomassen, EAJ; Abrahams, JP; Goosen, N; Pannu, NS
Author(s) at UniBasel Abrahams, Jan Pieter
Year 2008
Title 3C0S: UVDE 3 metals
Journal Worldwide Protein Data Bank
Pages / Article-Number 3C0S
Keywords Hydrolase
Mesh terms Science & TechnologyLife Sciences & BiomedicineBiochemistry & Molecular BiologyBiochemistry & Molecular Biology
Abstract

UV damage endonuclease is a DNA repair enzyme that can both recognize damage such as UV lesions and introduce a nick directly 5' to them. Recently, the crystal structure of the enzyme from Thermus thermophilus was solved. In the electron density map of this structure, unexplained density near the active site was observed at the tip of Lys229. Based on this finding, it was proposed that Lys229 is post-translationally modified. In this article, we give evidence that this modification is a carboxyl group. By combining activity assays and X-ray crystallography on several point mutants, we show that the carboxyl group assists in metal binding required for catalysis by donating negative charge to the metal-coordinating residue His231. Moreover, functional and structural analysis of the K229R mutant reveals that if His231 shifts away, an increased activity results on both damaged and undamaged DNA. Taken together, the results show that T. thermophilus ultraviolet damage endonuclease is carboxylated and the modified lysine is required for proper catalysis and preventing increased incision of undamaged DNA.

Full Text on edoc
Digital Object Identifier DOI 10.2210/pdb3c0s/pdb
ISI-Number DRCI:DATA2012140001768350
Document type (ISI) Data set
   

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