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A novel approximation method of CTF amplitude correction for 3D single particle reconstruction
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 4530984
Author(s) Jiang, Linhua; Liu, Zunfeng; Georgieva, Dilyana; Kuil, Maxim E.; Abrahams, Jan Pieter
Author(s) at UniBasel Abrahams, Jan Pieter
Year 2010
Title A novel approximation method of CTF amplitude correction for 3D single particle reconstruction
Journal Ultramicroscopy
Volume 110
Number 4
Pages / Article-Number 350-358
Abstract The typical resolution of three-dimensional reconstruction by cryo-EM single particle analysis is now being pushed up to and beyond the nanometer scale. Correction of the contrast transfer function (CTF) of electron microscopic images is essential for achieving such a high resolution. Various correction methods exist and are employed in popular reconstruction software packages. Here, we present a novel approximation method that corrects the amplitude modulation introduced by the contrast transfer function by convoluting the images with a piecewise continuous function. Our new approach can easily be implemented and incorporated into other packages. The implemented method yielded higher resolution reconstructions with data sets from both highly symmetric and asymmetric structures. It is an efficient alternative correction method that allows quick convergence of the 3D reconstruction and has a high tolerance for noisy images, thus easing a bottleneck in practical reconstruction of macromolecules.
Publisher Elsevier
ISSN/ISBN 0304-3991
edoc-URL https://edoc.unibas.ch/75922/
Full Text on edoc No
Digital Object Identifier DOI 10.1016/j.ultramic.2010.01.011
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/20163916
ISI-Number WOS:000276290200011
Document type (ISI) Article
 
   

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