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Imaging protein three-dimensional nanocrystals with cryo-EM
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 4530972
Author(s) Nederlof, Igor; Li, Yao Wang; van Heel, Marin; Abrahams, Jan Pieter
Author(s) at UniBasel Abrahams, Jan Pieter
Year 2013
Title Imaging protein three-dimensional nanocrystals with cryo-EM
Journal Acta Crystallographica. Section D, Biological Crystallography
Volume 69
Number Pt 5
Pages / Article-Number 852-9
Mesh terms Science & TechnologyLife Sciences & BiomedicinePhysical SciencesBiochemical Research MethodsBiochemistry & Molecular BiologyBiophysicsCrystallographyBiochemistry & Molecular BiologyBiophysicsCrystallography
Abstract Flash-cooled three-dimensional crystals of the small protein lysozyme with a thickness of the order of 100 nm were imaged by 300 kV cryo-EM on a Falcon direct electron detector. The images were taken close to focus and to the eye appeared devoid of contrast. Fourier transforms of the images revealed the reciprocal lattice up to 3 angstrom resolution in favourable cases and up to 4 angstrom resolution for about half the crystals. The reciprocal-lattice spots showed structure, indicating that the ordering of the crystals was not uniform. Data processing revealed details at higher than 2 angstrom resolution and indicated the presence of multiple mosaic blocks within the crystal which could be separately processed. The prospects for full three-dimensional structure determination by electron imaging of protein three-dimensional nanocrystals are discussed.
Publisher Munksgaard
ISSN/ISBN 0907-4449
edoc-URL https://edoc.unibas.ch/75914/
Full Text on edoc No
Digital Object Identifier DOI 10.1107/S0907444913002734
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/23633595
ISI-Number 000318240200020
Document type (ISI) Journal Article
 
   

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