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The influence of tRNA located at the P-site on the turnover of EF-Tu·GTP on ribosomes
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 4530742
Author(s) Abrahams, Jan Pieter; Acampo, J. J. C.; Kraal, B.; Bosch, L.
Author(s) at UniBasel Abrahams, Jan Pieter
Year 1991
Title The influence of tRNA located at the P-site on the turnover of EF-Tu·GTP on ribosomes
Journal Biochimie
Volume 73
Number 7-8
Pages / Article-Number 1089-1092
Keywords ELONGATION FACTOR-TU; GTPASE ACTIVITY; RIBOSOME; P-SITE; RIBOSOME EF TU INTERACTION
Mesh terms Science & TechnologyLife Sciences & BiomedicineBiochemistry & Molecular BiologyBiochemistry & Molecular Biology
Abstract The turnover of EF-Tu.GTP on poly-U programmed ribosomes was measured both in the presence and in the absence of N-acetylated Phe-tRNA(Phe) at the P-site. The reaction was uncoupled from protein synthesis by omitting Phe-tRNA(Phe) at the A-site. In this reaction, the ribosome can be considered as an enzyme catalysing the transition of EF-Tu.GTP to EF-Tu.GDP. A constant EF-Tu.GTP concentration is maintained by regenerating GDP to GTP at the expense of phosphoenolpyruvate by pyruvate kinase. The rate constants are determined using a procedure which corrects for the reduction in specific activity of GTP due to regeneration of the nucleotide. Ribosomes with an occupied P-site are more efficient in stimulating the GTPase of EF-Tu.GTP than ribosomes with an empty P-site. The data suggest that this is mainly caused by an increased affinity of EF-Tu.GTP for ribosomes with a filled P-site rather than by an enhanced reactivity of the GTPase centre.
Publisher Elsevier
ISSN/ISBN 0300-9084 ; 1638-6183
edoc-URL https://edoc.unibas.ch/75859/
Full Text on edoc No
Digital Object Identifier DOI 10.1016/0300-9084(91)90150-Y
ISI-Number 1991GF34800025
Document type (ISI) Article
 
   

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