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Structure-activity relationship in pyrazolo[4,3-c]pyridines, first inhibitors of PEX14-PEX5 protein-protein interaction with trypanocidal activity
Journal
Journal of medicinal chemistry
Volume
63
Number
2
Pages / Article-Number
847-879
Abstract
Trypanosoma; protists are pathogens leading to a spectrum of devastating infectious diseases. The range of available chemotherapeutics against; Trypanosoma; is limited, and the existing therapies are partially ineffective and cause serious adverse effects. Formation of the PEX14-PEX5 complex is essential for protein import into the parasites' glycosomes. This transport is critical for parasite metabolism and failure leads to mislocalization of glycosomal enzymes, with fatal consequences for the parasite. Hence, inhibiting the PEX14-PEX5 protein-protein interaction (PPI) is an attractive way to affect multiple metabolic pathways. Herein, we have used structure-guided computational screening and optimization to develop the first line of compounds that inhibit PEX14-PEX5 PPI. The optimization was driven by several X-ray structures, NMR binding data, and molecular dynamics simulations. Importantly, the developed compounds show significant cellular activity against; Trypanosoma; , including the human pathogen; Trypanosoma brucei gambiense; and; Trypanosoma cruzi; parasites.
