Data Entry: Please note that the research database will be replaced by UNIverse by the end of October 2023. Please enter your data into the system https://universe-intern.unibas.ch. Thanks

Login for users with Unibas email account...

Login for registered users without Unibas email account...

 
Translational regulation during zebrafish embryogenesis (EMBO Fellowship Madalena Pinto)
Third-party funded project
Project title Translational regulation during zebrafish embryogenesis (EMBO Fellowship Madalena Pinto)
Principal Investigator(s) Schier, Alexander
Project Members Madeira Reimão Pinto, Madalena
Organisation / Research unit Departement Biozentrum / Cell and Developmental Biology (Schier)
Department Departement Biozentrum / Cell and Developmental Biology (Schier)
Project Website http://schierlab.biozentrum.unibas.ch
Project start 01.01.2020
Probable end 31.12.2021
Status Completed
Abstract

Following fertilization, metazoan embryos are transcriptionally silent, and embryogenesis is controlled by maternally deposited factors. Developmental progression requires the synthesis of new mRNAs and proteins in a coordinated fashion. Many post­transcriptional mechanisms regulate the fate of maternal mRNAs, but it is less understood how translational control shapes early embryogenesis. Protein synthesis is primarily regulated at the translation initiation step by elements within the 5' untranslated region (UTR) of the mRNA. The goal of this project is to uncover the 5'UTR rules for mRNA translational regulation during zebrafish embryogenesis.

Aim 1: Distinct mRNAs display different translation efficiencies during embryogenesis. For example, ribosomal protein (RP) mRNAs remain translationally repressed during early stages of zebrafish development. How this repression is achieved and its biological significance remain unknown. I hypothesize that the vertebrate­conserved TOP motif, an oligopyrimidine tract characteristic to the 5'UTR of RP mRNAs, mediates their translational regulation during zebrafish embryogenesis. I aim to characterize the role of the TOP motif in RP mRNA translational regulation and identify the trans­acting factor(s) that binds to it during embryogenesis.

Aim 2: Currently, we lack a functional understanding of 5'UTR sequence composition in regulating mRNA translation during embryogenesis. By coupling an in vivo massively parallel reporter assay of annotated zebrafish 5'UTRs to polysome profiling, I will systematically assess the contribution of 5'UTR sequences to mRNA translational regulation during zebrafish development. These studies will identify novel functional 5'UTR elements that regulate the dynamics of mRNA translation during zebrafish embryogenesis.

Financed by Foreign Governmental Research Agencies
   

MCSS v5.8 PRO. 0.410 sec, queries - 0.000 sec ©Universität Basel  |  Impressum   |    
28/03/2024