In vitro reconstitution of bacterial DMSP biosynthesis

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In vitro reconstitution of bacterial DMSP biosynthesis

JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)

ID	4527745
Author(s)	Liao, Cangsong; Seebeck, Florian Peter
Author(s) at UniBasel	Seebeck, Florian Peter
Year	2019
Title	In vitro reconstitution of bacterial DMSP biosynthesis
Journal	Angew. Chemie Int. Ed
Volume	58
Number	11
Pages / Article-Number	3553-3556
Keywords	carbon–sulfur bond, DMSP, methyltransferase, pyridoxal phosphate, S-Methylmethionine
Abstract	Dimethylsulfoniopropionate (DMSP) is one of the most abundant sulfur metabolites in marine environments. The biosynthesis of DMSP and its degradation to dimethylsulfide are important links in the planetary sulfur cycle. Herein, the first complete description of a DMSP biosynthetic pathway is provided by the in vitro reconstitution of four enzymes from Streptomyces mobaraensis. The isolation of DMSP from S. mobaraensis cells grown at high salinity confirmed that this actinobacterium is indeed isaDMSP-producing organism. The described DMSP biosynthesis follows the same route as that previously described for angiosperm plants. Despite this chemical congruence, limited sequence similarity between plant and bacterial enzymes suggests that the two biosynthetic activities emerged by convergent evolution.
Publisher	WILEY-V C H VERLAG GMBH
edoc-URL	https://edoc.unibas.ch/75088/
Full Text on edoc	No
Digital Object Identifier DOI	10.1002/anie.201814662
ISI-Number	WOS:000460318200047
Document type (ISI)	Article

02/05/2024

Research Database / FORSCHUNGSDATENBANK