A convergent and essential interneuron pathway for Mauthner-cell-mediated escapes
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
ID 4519621
Author(s) Lacoste, Alix M. B.; Schoppik, David; Robson, Drew N.; Haesemeyer, Martin; Portugues, Ruben; Li, Jennifer M.; Randlett, Owen; Wee, Caroline L.; Engert, Florian; Schier, Alexander F.
Author(s) at UniBasel Schier, Alexander
Year 2015
Title A convergent and essential interneuron pathway for Mauthner-cell-mediated escapes
Journal Current biology : CB
Volume 25
Number 11
Pages / Article-Number 1526-1534
Mesh terms Animals; Animals, Genetically Modified; Escape Reaction, physiology; Interneurons, physiology; Zebrafish, physiology
Abstract The Mauthner cell (M-cell) is a command-like neuron in teleost fish whose firing in response to aversive stimuli is correlated with short-latency escapes [1-3]. M-cells have been proposed as evolutionary ancestors of startle response neurons of the mammalian reticular formation [4], and studies of this circuit have uncovered important principles in neurobiology that generalize to more complex vertebrate models [3]. The main excitatory input was thought to originate from multisensory afferents synapsing directly onto the M-cell dendrites [3]. Here, we describe an additional, convergent pathway that is essential for the M-cell-mediated startle behavior in larval zebrafish. It is composed of excitatory interneurons called spiral fiber neurons, which project to the M-cell axon hillock. By in vivo calcium imaging, we found that spiral fiber neurons are active in response to aversive stimuli capable of eliciting escapes. Like M-cell ablations, bilateral ablations of spiral fiber neurons largely eliminate short-latency escapes. Unilateral spiral fiber neuron ablations shift the directionality of escapes and indicate that spiral fiber neurons excite the M-cell in a lateralized manner. Their optogenetic activation increases the probability of short-latency escapes, supporting the notion that spiral fiber neurons help activate M-cell-mediated startle behavior. These results reveal that spiral fiber neurons are essential for the function of the M-cell in response to sensory cues and suggest that convergent excitatory inputs that differ in their input location and timing ensure reliable activation of the M-cell, a feedforward excitatory motif that may extend to other neural circuits.
Publisher CELL PRESS
ISSN/ISBN 1879-0445
URL https://www.sciencedirect.com/science/article/pii/S0960982215004765
edoc-URL https://edoc.unibas.ch/74127/
Full Text on edoc No
Digital Object Identifier DOI 10.1016/j.cub.2015.04.025
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/25959971
ISI-Number WOS:000355556600030
Document type (ISI) Article

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