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PDMS-PMOXA-Nanoparticles Featuring a Cathepsin B-Triggered Release Mechanism
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 4513252
Author(s) Ehrsam, Daniel; Porta, Fabiola; Hussner, Janine; Seibert, Isabell; Meyer Zu Schwabedissen, Henriette E.
Author(s) at UniBasel Meyer zu Schwabedissen, Henriette
Hussner, Janine
Seibert, Isabell
Porta, Fabiola
Ehrsam, Daniel
Year 2019
Title PDMS-PMOXA-Nanoparticles Featuring a Cathepsin B-Triggered Release Mechanism
Journal Materials
Volume 12
Number 17
Pages / Article-Number 2836
Keywords PDMS-PMOXA; cancer; cathepsin B; enzyme-triggered-release; nanoparticles; ovarian cancer; paclitaxel
Abstract It was our intention to develop cathepsin B-sensitive nanoparticles for tumor-site-directed release. These nanoparticles should be able to release their payload as close to the tumor site with a decrease of off-target effects in mind. Cathepsin B, a lysosomal cysteine protease, is associated with premalignant lesions and invasive stages of cancer. Previous studies have shown cathepsin B in lysosomes and in the extracellular matrix. Therefore, this enzyme qualifies as a trigger for such an approach.; Poly(dimethylsiloxane)-b-poly(methyloxazoline) (PDMS-PMOXA) nanoparticles loaded with paclitaxel were formed by a thin-film technique and standard coupling reactions were used for surface modifications. Despite the controlled release mechanism, the physical properties of the herein created nanoparticles were described. To characterize potential in vitro model systems, quantitative polymerase chain reaction and common bioanalytical methods were employed.; Stable paclitaxel-loaded nanoparticles with cathepsin B digestible peptide were formed and tested on the ovarian cancer cell line OVCAR-3. These nanoparticles exerted a pharmacological effect on the tumor cells suggesting a release of the payload.
Publisher MDPI
ISSN/ISBN 1996-1944
URL https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6747961
edoc-URL https://edoc.unibas.ch/71915/
Full Text on edoc No
Digital Object Identifier DOI 10.3390/ma12172836
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/31484396
Document type (ISI) Journal Article
 
   

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