Anti-Fas/CD95 and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) differentially regulate apoptosis in normal and neoplastic human basophils
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 4507573
Author(s) Förster, Anja; Falcone, Franco H; Gibbs, Bernhard F; Preussner, Liane M; Fiebig, Britta S; Altunok, Hülya; Seeger, Jens M; Cerny-Reiterer, Sabine; Rabenhorst, Anja; Papenfuss, Kerstin; Valent, Peter; Kashkar, Hamid; Hartmann, Karin
Author(s) at UniBasel Hartmann, Karin
Year 2013
Title Anti-Fas/CD95 and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) differentially regulate apoptosis in normal and neoplastic human basophils
Journal Leukemia & Lymphoma
Volume 54
Number 4
Pages / Article-Number 835-842
Mesh terms Antibodies, Monoclonal, pharmacology; Apoptosis, drug effects; Basophils, drug effects, metabolism, pathology; Cell Line; Cell Survival, drug effects; Cyclohexanes; Humans; Neoplasms, metabolism, pathology; Receptors, TNF-Related Apoptosis-Inducing Ligand, metabolism; TNF-Related Apoptosis-Inducing Ligand, pharmacology; fas Receptor, antagonists & inhibitors, metabolism
Abstract

Basophilia is associated with allergic and parasitic diseases and advanced chronic myeloid leukemia. In the present study, we characterized the expression and function of the death receptors Fas/CD95 and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptors in basophils from healthy donors compared to neoplastic basophils. Peripheral blood basophils obtained from healthy donors (HD-PBB) and from patients with chronic myeloid leukemia (CML-PBB) were found to express high levels of Fas/CD95 and low levels of TRAIL-R2, whereas the basophil-like chronic myeloid leukemia cell line KU-812 expressed significant levels of TRAIL-R1 and TRAIL-R2. HD-PBB underwent apoptosis in response to anti-Fas/CD95, but showed resistance to TRAIL, unless they were co-treated with actinomycin D. Interestingly, CML-PBB and KU-812 cells exhibited the opposite response pattern with resistance to anti-Fas/CD95, but significant susceptibility to TRAIL-induced apoptosis. Our data show that anti-Fas/CD95 and TRAIL differentially regulate apoptosis of normal and neoplastic human basophils, which may direct the development of novel therapeutic strategies.

ISSN/ISBN 1029-2403
Full Text on edoc
Digital Object Identifier DOI 10.3109/10428194.2012.731600
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/22989017
   

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