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A map of the interactome network of the metazoan C. elegans
Journal
Science
Volume
303
Number
5657
Pages / Article-Number
540-543
Mesh terms
Animals; Caenorhabditis elegans, genetics, metabolism; Caenorhabditis elegans Proteins, genetics, metabolism; Computational Biology; Evolution, Molecular; Genes, Helminth; Genomics; Open Reading Frames; Phenotype; Protein Binding; Proteome, metabolism; Transcription, Genetic; Two-Hybrid System Techniques
Abstract
To initiate studies on how protein-protein interaction (or "interactome") networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with a subset of metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated the overall quality of this Y2H data set. Together with already described Y2H interactions and interologs predicted in silico, the current version of the Worm Interactome (WI5) map contains approximately 5500 interactions. Topological and biological features of this interactome network, as well as its integration with phenome and transcriptome data sets, lead to numerous biological hypotheses.
Publisher
American Association for the Advancement of Science
