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A link between RNA interference and nonsense-mediated decay in Caenorhabditis elegans
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 4506872
Author(s) Domeier, Mary Ellen; Morse, Daniel P.; Knight, Scott W.; Portereiko, Michael; Bass, Brenda L.; Mango, Susan E.
Author(s) at UniBasel Mango, Susan Elizabeth
Year 2000
Title A link between RNA interference and nonsense-mediated decay in Caenorhabditis elegans
Journal Science
Volume 289
Number 5486
Pages / Article-Number 1928-1930
Mesh terms Adenosine Deaminase, metabolism; Alleles; Animals; Caenorhabditis elegans, genetics, metabolism; Caenorhabditis elegans Proteins; Gene Silencing; Helminth Proteins, genetics, metabolism; Mutation; Myosin Heavy Chains, genetics, metabolism; Nonmuscle Myosin Type IIB; Phosphoproteins, genetics, metabolism; RNA Stability; RNA, Double-Stranded, metabolism, pharmacology; RNA, Helminth, metabolism; Reverse Transcriptase Polymerase Chain Reaction
Abstract Double-stranded RNA (dsRNA) inhibits expression of homologous genes by a process involving messenger RNA degradation. To gain insight into the mechanism of degradation, we examined how RNA interference is affected by mutations in the smg genes, which are required for nonsense-mediated decay. For three of six smg genes tested, mutations resulted in animals that were initially silenced by dsRNA but then recovered; wild-type animals remained silenced. The levels of target messenger RNAs were restored during recovery, and RNA editing and degradation of the dsRNA were identical to those of the wild type. We suggest that persistence of RNA interference relies on a subset of smg genes.
Publisher American Association for the Advancement of Science
ISSN/ISBN 0036-8075 ; 1095-9203
edoc-URL https://edoc.unibas.ch/70572/
Full Text on edoc No
Digital Object Identifier DOI 10.1126/science.289.5486.1928
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/10988072
ISI-Number WOS:000089355800044
Document type (ISI) Article
 
   

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28/03/2024