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Two-dimensional; 1; H and; 1; H-detected NMR study of a heterogeneous biocatalyst using fast MAS at high magnetic fields
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 4494441
Author(s) Varghese, Sabu; Halling, Peter J.; Häussinger, Daniel; Wimperis, Stephen
Author(s) at UniBasel Häussinger, Daniel
Year 2018
Title Two-dimensional; 1; H and; 1; H-detected NMR study of a heterogeneous biocatalyst using fast MAS at high magnetic fields
Journal Solid state nuclear magnetic resonance
Volume 92
Pages / Article-Number 7-11
Abstract Nuclear magnetic resonance (NMR) is a powerful tool for investigating atomic-scale structure in heterogeneous or composite materials where long-range order is absent. In this work solid-state; 1; H and; 1; H-detected NMR experiments were performed with fast magic angle spinning (ν; R; = 75 kHz) and at high magnetic fields (B; 0; = 20 T) and used to gain structural insight into a heterogeneous biocatalyst consisting of an enzyme, human carbonic anhydrase II (hCA II), covalently immobilized on epoxy-functionalized silica. Two-dimensional; 1; H-; 1; H NOESY-type correlation experiments were able to provide information on; 1; H environments in silica, epoxy-silica and the immobilized enzyme. Two distinct signals originating from water protons were observed: water associated with the surface of the silica and the water associated with the immobilized enzyme. Additional two-dimensional; 1; H-; 1; H double-single quantum (DQ-SQ) correlation experiments suggested that the immobilized enzyme is not in close contact with the silica surface. Most significantly, comparison of two-dimensional; 1; H-; 15; N spectra of the immobilized enzyme and the solution-state enzyme confirmed that the structural integrity of the protein is well preserved upon covalent immobilization.
Publisher Elsevier
ISSN/ISBN 0926-2040 ; 1527-3326
edoc-URL https://edoc.unibas.ch/68484/
Full Text on edoc Available
Digital Object Identifier DOI 10.1016/j.ssnmr.2018.03.003
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/29587153
ISI-Number 000432597900002
 
   

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