A surface-induced asymmetric program promotes tissue colonization by Pseudomonas aeruginosa
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 4487722
Author(s) Laventie, Benoît-Joseph; Sangermani, Matteo; Estermann, Fabienne; Manfredi, Pablo; Planes, Rémi; Hug, Isabelle; Jaeger, Tina; Meunier, Etienne; Broz, Petr; Jenal, Urs;
Author(s) at UniBasel Jenal, Urs
Laventie, Benoit-Joseph
Manfredi, Pablo
Estermann, Fabienne
Sangermani, Matteo
Hug, Isabelle
Jaeger, Tina
Year 2019
Title A surface-induced asymmetric program promotes tissue colonization by Pseudomonas aeruginosa
Journal Cell Host & Microbe (accepted)
Volume 25
Number 1
Pages / Article-Number 140-152
Keywords Surface sensing, Flagella, Type IV pili, C-di-GMP, Second messenger, Asymmetric division, Tissue colonization, Pseudomonas aeruginosa, Virulence
Abstract

The opportunistic human pathogen Pseudomonas aeruginosa effectively colonizes host epithelia using pili as primary adhesins. Here we uncover a surface-specific asymmetric virulence program that greatly enhances P. aeruginosa host colonization. We show that when P. aeruginosa encounters surfaces, the concentration of the second messenger c-di-GMP increases within a few seconds. This leads to surface adherence and virulence induction by stimulating pili assembly through the activation of the c-di-GMP receptor FimW. Surface attached bacteria divide asymmetrically generating a piliated, surface committed progeny and a flagellated, motile offspring that leaves the surface to colonize distant sites. Cell differentiation is driven by a phosphodiesterase that asymmetrically positions to the flagellated pole thereby maintaining c-di-GMP levels low in the motile offspring. Infection experiments demonstrate that cellular asymmetry strongly boosts infection spread and tissue damage. Thus, P. aeruginosa promotes surface colonization and infection transmission through a cooperative virulence program that we termed Touch-Seed-and-Go.

Publisher CellPress
ISSN/ISBN 1931-3128
URL https://www.cell.com/cell-host-microbe
Full Text on edoc
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/30581112
   

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